FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462563
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG29590.6396966467270404No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT19130.41356528732302406No Hit
ATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCACAT19050.4118357931784427No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18190.39324373112419286No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15210.32882007423853615No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA14580.31520030784995773No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14220.3074175841993415No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAATTTTCTAGGGGGAGTACCCACG14160.30612046359090545No Hit
GTTCAGGATAATGTGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA13160.28450178678363813No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12140.26245073644022543No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11570.25012809066008307No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11390.24623672883477493No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11010.23802163164801335No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10920.23607595073535928No Hit
GTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAA10900.23564357719921394No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10890.23542739043114128No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTGTGAATAATGTCCTCCTGT10820.23391408305463257No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTCAT10380.22440186525943492No Hit
GTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGTACAATA10000.21618676807267334No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCGCCATA9880.21359252685580124No Hit
CTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCT9590.20732311058169373No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACAT9260.2001889472352955No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT9260.2001889472352955No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9020.19500046480155137No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8790.1900281691358799No Hit
GGTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGTACAAT8680.18765011468708045No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8640.18678536761478978No Hit
TTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAATTTTCGAAAAC8570.18527206023828105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8320.1798673910364642No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8110.17532746890693807No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGAAAATTGCCTGTCA8020.17338178799428403No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7910.17100373354548462No Hit
CAATAGGTCTATTGACAGGCAATTTTCGAAAACATTGTTTGATTTTTTGT7900.17078754677741192No Hit
GGATAATGTGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGTTT7830.16927423940090322No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT7710.16667999818403115No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATGTGGTTTAATGCC7550.16322100989486837No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7340.15868108776534223No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7300.15781634069305153No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7270.15716778038883353No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCGCCATATT7260.15695159362076083No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAAACTTACCAAATAT7200.1556544730123248No Hit
CTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTCATATTCATTTACAGG7130.1541411656358161No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7070.15284404502738005No Hit
GTTCTATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6720.14527750814483648No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6680.1444127610725458No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6660.14398038753640044No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA6640.1435480140002551No Hit
GAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGT6630.1433318272321824No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6400.13835953156651093No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6310.1364138506538569No Hit
GTCAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT6250.13511673004542082No Hit
TAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTGATAG6220.13446816974120282No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT6190.1338196094369848No Hit
CATCCACTACCAGCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAA6140.13273867559662145No Hit
ATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTT6100.13187392852433075No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTCTATTCTTCTACTG6060.13100918145204005No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6010.12992824761167668No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA6000.12971206084360398No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5930.1281987534670953No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5920.1279825666990226No Hit
ACATTATTCACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAA5920.1279825666990226No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCACAAGACCGCCGTG5890.1273340063948046No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5890.1273340063948046No Hit
GATTAACTGCATGTTCAGGATAATGTGGTTTAATGCCTTTGTCCAAGGGC5870.12690163285865927No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5850.1264692593225139No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTCTATTCTTCTACTGTACCTGTC5760.12452357840985985No Hit
CATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5760.12452357840985985No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5640.12192933719298778No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACGTTTCC5560.12019984304840639No Hit
ATTTACAGGAGGACATTATTCACAGATGTCAACAATTTGTGGGCCCTCTT5540.11976746951226104No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5510.11911890920804301No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5500.11890272243997033No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTA5500.11890272243997033No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC5500.11890272243997033No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5490.11868653567189767No Hit
GTATCCAGTTGGCAGCACACCCTAGCAGCCATGGAAAGGAGGTGTATTTC5460.11803797536767964No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5460.11803797536767964No Hit
CAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5440.11760560183153429No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACA5440.11760560183153429No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5430.11738941506346164No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC5400.1167408547592436No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5370.1160922944550256No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG5360.1158761076869529No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5310.11479517384658955No Hit
CTCCAAAAGACACCAAGTACTCAAGAACAGTATCTCTTCCAAACGTAAGA5290.11436280031044418No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5280.11414661354237153No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTCTATTC5280.11414661354237153No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5230.11306567970200815No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT5230.11306567970200815No Hit
CATTTACAGGAGGACATTATTCACAGATGTCAACAATTTGTGGGCCCTCT5200.11241711939779014No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTCCCTGACTGCCGATTG5170.11176855909357211No Hit
ATTCACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAG5080.10982287818091807No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5050.10917431787670004No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4930.10658007665982797No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4910.10614770312368262No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4890.10571532958753727No Hit
CCTCTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT4870.10528295605139193No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4860.10506676928331925No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCT4850.10485058251524658No Hit
TAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA4820.10420202221102855No Hit
GCTAGGTTCTATCCTAAACTTACCAAATATTTGCCCTTGGACAAAGGCAT4800.10376964867488321No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTGACAGGCAATTTTCG4790.10355346190681053No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4780.10333727513873786No Hit
GTCCAAGGGCAAATATTTGGTAAGTTTAGGATAGAACCTAGCAGGCATAA4780.10333727513873786No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4770.10312108837066518No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4660.10074303392186579No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGAGTA4650.1005268471537931No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4640.10031066038572044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)