FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506592
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48270.9528377866211862No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT19120.37742404143768554No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15490.3057687448676647No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14810.2923457141052366No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTATTACAAAGATC13510.2666840376476533No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGA10920.21555808224369907No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT10200.20134546143642224No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGT9080.1792369401806582No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8930.17627597751247553No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8620.170156654664898No Hit
CGTTAATATGGGCCTAAAAATCAGACAAATATTGTGGTTTCACATTTCCT8590.16956446213126145No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA8570.1691696671088371No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8520.1681826795527762No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8140.1606815741267134No Hit
GTGGATATCCTGCTTTAATGCCGTTATATGCATGTATACAAGCAAAACAG8100.1598919840818647No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7980.15752321394731855No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7440.1468637483418609No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7200.14212620807276863No Hit
GTATACATGCATATAACGGCATTAAAGCAGGATATCCACATTGCGTGAAA6940.13699387278125197No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6760.13344071757943277No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6730.13284852504579622No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6650.1312693449560988No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6620.13067715242246228No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6560.1294927673551892No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6350.12534741961973342No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6330.12495262459730908No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT6270.123768239530036No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6220.1227812519739751No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6180.1219916619291264No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6160.12159686690670203No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6150.12139946939548987No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6150.12139946939548987No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6120.12080727686185333No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA6070.11982028930579243No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCCAGGGGGAACACCCGTG6030.11903069926094373No Hit
GTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTT6020.11883330174973154No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT5990.118241109216095No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5950.11745151917124629No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGACA5920.11685932663760976No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5900.1164645316151854No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5870.11587233908154887No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5860.11567494157033668No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5750.11350356894700273No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5730.11310877392457835No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5730.11310877392457835No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5660.11172699134609312No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTC5600.11054260627882002No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA5500.10856863116669825No Hit
ATGTGGATATCCTGCTTTAATGCCGTTATATGCATGTATACAAGCAAAAC5420.10698945107700082No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5400.10659465605457646No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5370.10600246352093994No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5370.10600246352093994No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5260.10383109089760595No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5150.101659718274272No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5120.10106752574063546No Hit
AGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5070.10008053818457457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)