FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506592
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47960.9467184637736088No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT19230.37959541406101954No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14560.28741077632493206No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13620.2688554102709873No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTATTACAAAGATC13340.26332827995704633No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT11810.233126460741583No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGA11160.22029562251279136No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGT8630.17035405217611016No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8530.1683800770639884No Hit
CGTTAATATGGGCCTAAAAATCAGACAAATATTGTGGTTTCACATTTCCT8520.1681826795527762No Hit
GTGGATATCCTGCTTTAATGCCGTTATATGCATGTATACAAGCAAAACAG8490.16759048701913964No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8400.16581390941823004No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA8370.16522171688459353No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8310.16403733181732044No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8080.15949718905944033No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8020.15831280399216727No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7610.15021950603246795No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7470.14745594087549743No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA6980.13778346282610068No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCCAGGGGGAACACCCGTG6520.12870317731034048No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6470.12771618975427956No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6440.12712399722064305No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6420.12672920219821868No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6390.12613700966458216No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6380.12593961215336996No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6310.12455782957488473No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6100.12041248183942896No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6060.11962289179458026No Hit
GTATACATGCATATAACGGCATTAAAGCAGGATATCCACATTGCGTGAAA6050.11942549428336807No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5880.11606973659276103No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5850.1154775440591245No Hit
GTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTT5810.1146879540142758No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5810.1146879540142758No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5740.11330617143579054No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTC5680.11212178636851747No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTTTCCCAAAAGTAAGACA5600.11054260627882002No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5590.11034520876760787No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5580.11014781125639568No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5530.1091608237003348No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5530.1091608237003348No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5520.10896342618912261No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5480.10817383614427388No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5400.10659465605457646No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5390.10639725854336428No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5350.10560766849851558No Hit
ATGTGGATATCCTGCTTTAATGCCGTTATATGCATGTATACAAGCAAAAC5300.10462068094245468No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5210.10284410334154506No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5090.10047533320699893No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5090.10047533320699893No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5070.10008053818457457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)