FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524000
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG25540.4874045801526718No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG23410.44675572519083967No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC22720.433587786259542No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTT22400.4274809160305344No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT20780.3965648854961832No Hit
GGATATCATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG20640.39389312977099233No Hit
ATCCTAAACGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT19580.3736641221374046No Hit
GTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG18880.3603053435114504No Hit
TCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA16910.32270992366412216No Hit
GGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15790.3013358778625954No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13880.2648854961832061No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT13790.26316793893129775No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA13180.2515267175572519No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13060.24923664122137404No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12800.24427480916030536No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT12800.24427480916030536No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT12580.2400763358778626No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC12540.23931297709923663No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA12400.2366412213740458No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG12250.2337786259541985No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA11890.22690839694656487No Hit
AGGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAAC11790.22499999999999998No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGA11650.22232824427480918No Hit
GAGGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAA11080.21145038167938932No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC10930.208587786259542No Hit
GATAATGTCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTGCCATT10910.208206106870229No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT10870.20744274809160307No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10850.20706106870229007No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10800.20610687022900764No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT10630.20286259541984733No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10530.20095419847328244No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC10350.19751908396946566No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCTTTC10240.19541984732824427No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA10210.1948473282442748No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGAACAAAGATC10190.19446564885496184No Hit
GAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA10140.1935114503816794No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9740.18587786259541986No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCACTTGAAAG9720.18549618320610686No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT9190.17538167938931296No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC8690.16583969465648854No Hit
ATGTTGTATTGGGGGCCAAATCTGTACAACACCTTGAATCCCTTTTTACC8620.16450381679389314No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8570.16354961832061068No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAATCTGTACAACACCTTGAA8530.16278625954198475No Hit
ATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAACCTAGCAGGCATGA8530.16278625954198475No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTTAG8370.15973282442748094No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8320.15877862595419848No Hit
CCTTTATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAACCTAGCAGG8220.1568702290076336No Hit
ATATGTTCCTGTGGCAAGGCACCCCAACTTCCAATTACATATCCCATGAA8110.15477099236641223No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8090.15438931297709924No Hit
CCTATAAATAGACCTATCGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8060.15381679389312977No Hit
ACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAACTGTAAGA8020.1530534351145038No Hit
GTTCTATCCTAAACGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8020.1530534351145038No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTGCCTTGCCACAG7850.1498091603053435No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7770.1482824427480916No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGA7710.14713740458015268No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7660.14618320610687024No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATT7640.14580152671755725No Hit
ATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAG7430.14179389312977098No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA7330.1398854961832061No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT6940.13244274809160306No Hit
AAATAGACCTATCGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6910.1318702290076336No Hit
ACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGG6880.1312977099236641No Hit
TTGACATACTTTCCAATCGATAGGTCTATTTATAGGAAGTTTCCGAAAAC6830.13034351145038167No Hit
TGATATCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTGCCATTCA6800.1297709923664122No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6720.1282442748091603No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCACTTGAA6630.1265267175572519No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6540.1248091603053435No Hit
CCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGACA6540.1248091603053435No Hit
CTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTA6380.12175572519083969No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6340.12099236641221374No Hit
GCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAAC6310.12041984732824429No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA6210.1185114503816794No Hit
AATCTGTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT6160.11755725190839696No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6160.11755725190839696No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT6100.11641221374045801No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAACACCTTGAATCCCTTTTTACC6090.11622137404580153No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC6030.11507633587786259No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5980.11412213740458015No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5850.11164122137404581No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGGTT5820.11106870229007633No Hit
TTCTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5790.11049618320610687No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT5760.10992366412213742No Hit
GTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCA5680.1083969465648855No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATG5650.10782442748091603No Hit
CAATTCTTTGACATACTTTCCAATCGATAGGTCTATTTATAGGAAGTTTC5620.10725190839694655No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5510.10515267175572521No Hit
ACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5460.10419847328244276No Hit
CAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT5410.10324427480916032No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5400.10305343511450382No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5400.10305343511450382No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5380.10267175572519084No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGT5330.1017175572519084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)