FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524000
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG24970.47652671755725196No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTT24670.47080152671755726No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG23030.43950381679389317No Hit
GTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG22770.43454198473282446No Hit
GGATATCATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG21200.40458015267175573No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC20310.38759541984732826No Hit
ATCCTAAACGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT18070.34484732824427483No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17860.34083969465648856No Hit
TCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA16230.3097328244274809No Hit
GGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15080.2877862595419847No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTT14600.2786259541984733No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT14240.27175572519083974No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA13990.2669847328244275No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13870.2646946564885496No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT13230.25248091603053435No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12570.23988549618320612No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12310.2349236641221374No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC12230.2333969465648855No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA12090.23072519083969464No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG12060.23015267175572518No Hit
GATAATGTCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTGCCATT11660.22251908396946565No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA11470.21889312977099237No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11360.216793893129771No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGA11330.21622137404580152No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGT11150.21278625954198474No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT10810.2062977099236641No Hit
GAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA10740.2049618320610687No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC10620.20267175572519086No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGAACAAAGATC10520.20076335877862597No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCTTTC10150.19370229007633588No Hit
AGGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAAC10010.19103053435114503No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9970.1902671755725191No Hit
GAGGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAA9860.1881679389312977No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9740.18587786259541986No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTTAG9680.18473282442748093No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA9550.18225190839694655No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9400.17938931297709923No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9060.17290076335877863No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCACTTGAAAG9040.17251908396946564No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC8990.1715648854961832No Hit
ATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAACCTAGCAGGCATGA8910.17003816793893128No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGA8720.16641221374045803No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT8680.16564885496183207No Hit
ATATGTTCCTGTGGCAAGGCACCCCAACTTCCAATTACATATCCCATGAA8430.16087786259541986No Hit
CCTTTATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAACCTAGCAGG8280.15801526717557252No Hit
CCTATAAATAGACCTATCGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8090.15438931297709924No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAATCTGTACAACACCTTGAA7950.15171755725190839No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATT7880.15038167938931296No Hit
ATGTTGTATTGGGGGCCAAATCTGTACAACACCTTGAATCCCTTTTTACC7840.14961832061068703No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7770.1482824427480916No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7580.14465648854961832No Hit
CAATTCTTTGACATACTTTCCAATCGATAGGTCTATTTATAGGAAGTTTC7550.14408396946564886No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7470.14255725190839696No Hit
TTGACATACTTTCCAATCGATAGGTCTATTTATAGGAAGTTTCCGAAAAC7370.14064885496183208No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA7260.1385496183206107No Hit
ATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAG7250.1383587786259542No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTGCCTTGCCACAG7240.1381679389312977No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7230.13797709923664123No Hit
TGATATCCTCTTTCAAGTGAATGTGAGGAAAAGAGGGAGTTTGCCATTCA7180.13702290076335877No Hit
AAATAGACCTATCGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG7170.1368320610687023No Hit
ACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAACTGTAAGA7080.13511450381679388No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7040.13435114503816795No Hit
GTTCTATCCTAAACGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC7020.13396946564885498No Hit
ACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGG6800.1297709923664122No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCACTTGAA6770.12919847328244277No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6750.12881679389312978No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6680.12748091603053435No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA6610.12614503816793893No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6490.12385496183206107No Hit
GTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATA6250.11927480916030535No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATG6240.11908396946564885No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT6240.11908396946564885No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6190.1181297709923664No Hit
GGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAA6190.1181297709923664No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA6190.1181297709923664No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6120.11679389312977098No Hit
CCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGACA6070.11583969465648854No Hit
GCCTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAAC5940.1133587786259542No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5820.11106870229007633No Hit
GTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGTTTAGGATAGAAC5750.10973282442748092No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5690.10858778625954199No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT5690.10858778625954199No Hit
AATCTGTACAACACCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT5630.10744274809160305No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC5570.10629770992366411No Hit
ATGTGGTATTGGGGGCCAAATCTGTACAACACCTTGAATCCCTTTTTACC5450.10400763358778627No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5440.10381679389312977No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5400.10305343511450382No Hit
GTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCA5390.10286259541984732No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5390.10286259541984732No Hit
TTCTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5360.10229007633587787No Hit
CTTCCACAGAGTATGCAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5360.10229007633587787No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGGTT5350.10209923664122138No Hit
CTTTCCAATCGATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTG5300.10114503816793893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)