FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362495
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12590.34731513538117764No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11580.3194526821059601No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT11450.31586642574380336No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11060.3051076566573332No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10510.28993503358667017No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10060.2775210692561277No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9800.2703485565318142No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9060.24993448185492212No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8650.23862398102042787No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8390.23145146829611443No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8380.2311756024221024No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8020.22124443095766838No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCATACTCCTTCTTTTCCTCAC7800.21517538172940318No Hit
CCTCAACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7500.2068994055090415No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTGTTGATAATATCCTCTTTC7330.20220968565083658No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACT7240.19972689278472808No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7100.1958647705485593No Hit
AGTCTGTACAACACCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT7040.19420957530448696No Hit
CTTTAATCCTGAGTGGCATACTCCTTCTTTTCCTCACATTCATTTGAAAG6990.1928302459344267No Hit
CTTAAAGGTGGAGAGATGGGAGTTGGCTGTCTTCCTGACTGCCGATTGGT6750.18620946495813737No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6720.18538186733610118No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6720.18538186733610118No Hit
GGATATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG6660.18372667209202884No Hit
ATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAA6660.18372667209202884No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT6650.1834508062180168No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6570.18124387922592036No Hit
GGATAAAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6490.1790369522338239No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6300.17379550062759488No Hit
GTATAAAGGGATTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC6210.17131270776148635No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6210.17131270776148635No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGC6110.1685540490213658No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6080.16772645139932965No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6070.1674505855253176No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5960.164416060911185No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5930.1635884632891488No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5900.16276086566711265No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5790.15972634105298006No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC5680.15669181643884741No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA5560.15338142595070278No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5500.15172623070663044No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5420.14951930371453398No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5420.14951930371453398No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5320.14676064497441346No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCATACTCCTTCTTTTC5240.144553717982317No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5110.14096746162016027No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5070.13986399812411207No Hit
CCTTTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACCAC4960.13682947350997945No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4920.13572601001393123No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACTC4900.1351742782659071No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4870.13434668064387095No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4860.13407081476985888No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4850.13379494889584684No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4810.13269148539979864No Hit
ATGCTGGGTCGTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC4770.1315880219037504No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4720.1302086925336901No Hit
CTGTAAGGTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4580.12634657029752136No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4580.12634657029752136No Hit
ATGTAAGACCTTAGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGC4570.1260707044235093No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4560.12579483854949725No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCGTCCAAATTACTTCCCACCC4470.12331204568338874No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC4450.12276031393536463No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4400.12138098456530434No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT4360.12027752106925614No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4340.11972578932123203No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4300.1186223258251838No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAG4290.11834645995117174No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAAGGAGTATGCC4270.11779472820314763No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4260.11751886232913558No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA4240.11696713058111147No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAACCTTACAGACCACCAAATGCC4190.1155878012110512No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4190.1155878012110512No Hit
GTACAACACCTTGAATCCCTTTATACCGCTATTACCAATTTTCTTTTGTC4160.11476020358901502No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4140.11420847184099091No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4140.11420847184099091No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4060.11200154484889446No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA4050.11172567897488242No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA4040.11144981310087036No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3990.11007048373081008No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA3990.11007048373081008No Hit
GGGTATATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATC3960.10924288610877392No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG3920.10813942261272569No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3920.10813942261272569No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACCACTGCTGGTGGCTCCAGTT3880.10703595911667747No Hit
ACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC3870.10676009324266543No Hit
GTTGTCAATATACCCTGAGCCTGAGGGCTCCATCCCAAAAGATTGCCGTG3820.10538076387260513No Hit
GGGTAAAGGTCCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3820.10538076387260513No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3800.10482903212458103No Hit
GCCTAGTCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC3790.10455316625056899No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTAAGGCA3780.10427730037655691No Hit
GTGTAAACAATATCTGGACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC3780.10427730037655691No Hit
ACCTTAGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGCGACGTGC3770.10400143450254487No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3770.10400143450254487No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3760.1037255686285328No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3760.1037255686285328No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3750.10344970275452076No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3700.10207037338446048No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3690.10179450751044841No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3680.10151864163643637No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGGCCTGGCGGGCGTTC3670.10124277576242431No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA3660.10096690988841225No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3640.10041517814038815No Hit
CCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC3630.10013931226637608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)