FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362495
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG13870.38262596725472076No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12980.3580739044676478No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11620.32055614560200835No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10530.29048676533469425No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10250.2827625208623567No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9240.2549000675871391No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8330.2297962730520421No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8240.2273134801859336No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8130.224278955571801No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7990.2204168333356322No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7750.2137960523593429No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTGTTGATAATATCCTCTTTC7540.20800286900508974No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7340.20248555152484862No Hit
CCTCAACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7190.1983475634146678No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCATACTCCTTCTTTTCCTCAC7180.1980716975406557No Hit
CTTTAATCCTGAGTGGCATACTCCTTCTTTTCCTCACATTCATTTGAAAG7180.1980716975406557No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACT6970.19227851418640257No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6650.1834508062180168No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT6470.1784852204857998No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6370.17572656174567924No Hit
CTTAAAGGTGGAGAGATGGGAGTTGGCTGTCTTCCTGACTGCCGATTGGT6350.17517482999765516No Hit
GGATATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACG6280.17324376887957077No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6200.1710368418874743No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6110.1685540490213658No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6060.16717471965130554No Hit
GTATAAAGGGATTCAAGGTGTTGTACAGACTTGGCCCCCAATACCACATC6030.16634712202926938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5950.16414019503717292No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5780.159450475178968No Hit
AGTCTGTACAACACCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT5720.15779527993489564No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5710.1575194140608836No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5600.15448488944675098No Hit
ATTATCAACAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAACGAAAA5550.1531055600766907No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5530.1525538283286666No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5530.1525538283286666No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5470.15089863308459428No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5380.14841584021848575No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGC5300.14620891322638935No Hit
GGATAAAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5280.14565718147836523No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5270.14538131560435316No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC5260.14510544973034112No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5220.1440019862342929No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5160.14234679099022055No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5090.14041572987213619No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5090.14041572987213619No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5060.1395881322501No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5050.13931226637608796No Hit
CTGTAAGGTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4910.13545014413991915No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAG4820.13296735127381068No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4790.13213975365177452No Hit
ATGCTGGGTCGTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAAC4750.1310362901557263No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA4700.129656960785666No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4640.12800176554159368No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4590.1266224361715334No Hit
CCTTTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACCAC4550.12551897267548517No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4520.12469137505344902No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAAGGAGTATGCC4520.12469137505344902No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCATACTCCTTCTTTTC4500.1241396433054249No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT4390.1211051186912923No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4380.12082925281728023No Hit
GTGTAAACAATATCTGGACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4340.11972578932123203No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4310.11889819169919585No Hit
GGGTAAAGGTCCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4200.11586366708506324No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC4190.1155878012110512No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4170.11503606946302708No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4110.11338087421895475No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCGTCCAAATTACTTCCCACCC4050.11172567897488242No Hit
TCCTTTGTCTACGTCCCGTCGGCGTTGAATCCCGCGGACGACCCGTCTCG4040.11144981310087036No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4010.11062221547883419No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4000.11034634960482215No Hit
CCCCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC3960.10924288610877392No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3960.10924288610877392No Hit
GGGTATATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATC3950.10896702023476185No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA3940.1086911543607498No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3930.10841528848673775No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3900.10758769086470159No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAACCTTACAGACCACCAAATGCC3890.10731182499068952No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA3890.10731182499068952No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3860.10648422736865336No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA3840.10593249562062924No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA3830.1056566297466172No Hit
GTACAACACCTTGAATCCCTTTATACCGCTATTACCAATTTTCTTTTGTC3810.10510489799859309No Hit
GCCTAGTCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC3810.10510489799859309No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3750.10344970275452076No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACCACTGCTGGTGGCTCCAGTT3750.10344970275452076No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3750.10344970275452076No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACTC3720.10262210513248457No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3700.10207037338446048No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA3700.10207037338446048No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTAAGGCA3670.10124277576242431No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAATCCCTTTATACC3670.10124277576242431No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3640.10041517814038815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)