FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479023
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42770.8928590067700298No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25830.539222542550149No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17700.3695020907138071No Hit
ATCCTAATCTTACCAAATACTTGCCCTTGGACAAAGGCATTAAACCGTAT13260.2768134306703436No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11900.24842230957594938No Hit
GATTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11000.2296340676752473No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10890.22733772699849486No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA10760.2246238698350601No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10140.2116808587479098No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAATACAATA10030.20938451807115732No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9810.20479183671765236No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9800.2045830784743113No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9650.20145170482419425No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAATACAAT9590.20019915536414745No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA9390.19602399049732477No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8570.17890581454335178No Hit
ATTCTATCCTAATCTTACCAAATACTTGCCCTTGGACAAAGGCATTAAAC8460.1766094738665993No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGATACTCCCTTAACTTCAT8430.17598319913657592No Hit
GTTTTAATACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA8350.17431313318984684No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA8330.17389561670316458No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAGTATTTGGTAAGATT8120.16951169359300075No Hit
GTTAAGGGAGTATCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC8110.16930293534965962No Hit
CTGTAAGAGGTCCCACATATTGTTGACATCTATCAATAATGTCCTCTTTT8110.16930293534965962No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAAT8090.16888541886297737No Hit
CATTTAAAAGAGGACATTATTGATAGATGTCAACAATATGTGGGACCTCT8000.16700659467290715No Hit
AGTTAAGGGAGTATCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA7690.16053508912933198No Hit
GTGTAAAAGGGGCAGCAAAGCCCAACAGACCCACAATTCGTTGACATACT7200.15030593520561644No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7020.14654828682547602No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6970.14550449560877035No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTATTAAAA6940.14487822087874697No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6920.14446070439206468No Hit
GTATTTGGTAAGATTAGGATAGAATCTAGCAGGCATAATTAATTTTAATC6710.14007678128190088No Hit
GTATTAAAACTCAAACAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT6710.14007678128190088No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTTCTGTACCTGTC6670.13924174830853633No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6630.1384067153351718No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6500.13569285817173704No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6490.1354840999283959No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6470.13506658344171366No Hit
GGAACATATTGTATTAAAACTCAAACAATGTTTTCGAAAATTGCCTGTAA6400.1336052757383257No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAGTATTTGGTA6340.1323527262782789No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC6300.13151769330491436No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGATACTCCCTTAAC6180.12901259438482077No Hit
GCTAGATTCTATCCTAATCTTACCAAATACTTGCCCTTGGACAAAGGCAT6130.1279688031681151No Hit
GGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6100.12734252843809168No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6080.12692501195140943No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6020.12567246249136263No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6010.1254637042480215No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5930.12379363830129242No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5910.12337612181461015No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5890.12295860532792789No Hit
CAATATGTGGGACCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5840.12191481411122221No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGC5750.12003598992115201No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5750.12003598992115201No Hit
CCCTTGGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA5700.11899219870444633No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5570.11627834154101159No Hit
CTTCTGTACCTGTCTTTAATTCTGAATGGCAAACTCCCTCCTTTCCTCAC5530.11544330856764708No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5530.11544330856764708No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5490.11460827559428254No Hit
CTTTAATTCTGAATGGCAAACTCCCTCCTTTCCTCACATTCATTTAAAAG5480.1143995173509414No Hit
CTTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCATTCAGAATTAAAG5470.11419075910760025No Hit
GTCCAAGGGCAAGTATTTGGTAAGATTAGGATAGAATCTAGCAGGCATAA5460.11398200086425914No Hit
GTTTAATGCCTTTGTCCAAGGGCAAGTATTTGGTAAGATTAGGATAGAAT5460.11398200086425914No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5410.11293820964755345No Hit
ATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAATACAATATGTT5320.11105938545748326No Hit
TTATTGATAGATGTCAACAATATGTGGGACCTCTTACAGTTAATGAAAAA5260.10980683599743646No Hit
GCTTTAGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG5260.10980683599743646No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5220.10897180302407192No Hit
ATACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5210.10876304478073077No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5190.10834552829404852No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5180.10813677005070738No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5140.10730173707734283No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5120.10688422059066058No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5050.10542291288727264No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTATCCCCAGCGTTTGGTTTTA4970.10375284694054356No Hit
ATGTGGGACCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG4940.10312657221052016No Hit
CAATCCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTT4940.10312657221052016No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4900.10229153923715564No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4800.10020395680374429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)