FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593714
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113671.9145581879490798No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA39590.6668193776801625No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTACTCCTTACATTTCAT21360.35976918179460143No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC20100.3385468424190772No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT17430.29357569469475203No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT15420.2597210104528443No Hit
CTCTAAGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14760.24860454697042683No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAT14710.2477623906460013No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13560.22839279518421327No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTCTGTATGAT13480.22704534506513235No Hit
GTATCACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC13300.22401358229720034No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13280.22367671976743012No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT12870.21677103790714047No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAATCTAGCAGG12780.21525515652317445No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12510.21070751237127638No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCTGTAATGTCAACGACCGACCT12210.205654574424723No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC11600.19538026726673113No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA10350.174326359156092No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGGGAGTCCAA9660.1627046018790192No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9590.1615255830248234No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTATTCTCCTCC9320.15697793887292535No Hit
CACTAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT9040.15226186345614218No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8960.15091441333706127No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCCAAATGTTA8560.14417716274165676No Hit
ACTACTGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG8430.1419875562981503No Hit
GTCTGTATCACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTT8140.137103049616482No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7930.13356599305389463No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7860.13238697419969886No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA7850.13221854293481375No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7720.13002893649130726No Hit
TTGACATACTTTCCAATCAATAGGCCGGTTGACAGGAAGTTTTCTAAAAC7670.1291867801668817No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGAATA7570.12750246751803057No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7420.12497599854475387No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATTAACCTATTCT7370.1241338422203283No Hit
AGTCTGTATCACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7340.12362854842567297No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG7330.12346011716078786No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7310.12312325463101761No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATT7210.12143894198216651No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7190.12110207945239626No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGATTTTATCC7160.12059678565774093No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7160.12059678565774093No Hit
ATGTCATTGGATGTTATGGATCATTGCCACAAGATCACATCATACAGAAA7000.11790188541957912No Hit
GATTATGCCTGCTAGATTTTATCCAAATGTTACCAAATATTTGCCATTGG6960.11722816036003868No Hit
ACTGTTGAGTTACTCTCGTTTTTGCCTGCTGACTTCTTTCCTTCAGTACG6830.1150385539165322No Hit
TCCTAATACAAAGATCATTAACCTATTCTCCTCCCCCAACTCCTCCCAGT6730.11335424126768107No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6690.11268051620814062No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6610.11133306608905971No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6570.11065934102951927No Hit
CTCTTGGACTCCCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6500.10948032217532348No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATGTTGGATCGTC6500.10948032217532348No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6500.10948032217532348No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC6500.10948032217532348No Hit
GTTATGGATCATTGCCACAAGATCACATCATACAGAAAATCAAAGATTGT6420.10813287205624256No Hit
CTCTAAGGCTTCCCGAAATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6210.1045958154936552No Hit
GGTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6210.1045958154936552No Hit
GTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6160.10375365916922963No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6150.10358522790434452No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6080.10240620905014872No Hit
GGCCGGTTGACAGGAAGTTTTCTAAAACAATCTTTGATTTTCTGTATGAT6070.1022377777852636No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGCAGGCAAAAACGAGA5980.10072189640129761No Hit
GTCTTTGGGCATACATTTAAACCCTCACAAAACAAAAAGATGGGGTTACT5980.10072189640129761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)