FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445489
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGA19220.431436017499871No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACATC15020.33715759536150164No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13290.29832386433784003No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10740.24108339375383006No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9640.21639142605092382No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9630.21616695361726104No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9580.2150445914489471No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8700.1952910172866221No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT8510.19102604104702922No Hit
CTCTATGGAAGGCGGGGATATTATATAAGAGAGAAACTACACATAGCGCC8430.18923026157772693No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC8390.1883323718430758No Hit
CAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAGCATTC8340.1872100096747619No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7820.17553744312429712No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7670.17217035661935537No Hit
CTTATATAATATCCCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGTTT7620.17104799445104144No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7460.16745643551243689No Hit
TCCCCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTACAAG7230.16229356953819285No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7000.15713070356394881No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTATTGTGGTTTCACATTTCTT6700.1503965305540653No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTCTGTATGCCTGTATT6540.14680497161546074No Hit
CTCTTATATAATATCCCCGCCTTCCATAGAGTGTGTAAATAATGTCTAGT6270.14074421590656558No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG5910.13266320829470538No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5700.12794928718778692No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5590.12548009041749628No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC5580.1252556179838335No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5570.12503114555017072No Hit
GTGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5530.12413325581551958No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC5250.11784802767296162No Hit
AATATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5150.11560330333633378No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5140.11537883090267101No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5070.11380752386703152No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5070.11380752386703152No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA4800.10774676815813633No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4770.10707335085714798No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4720.10595098868883407No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCTGTCCCAAGAATGTTT4670.10482862652052014No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC4640.10415520921953178No Hit
GCCTAGCAGCCATGGGAACGATGTATATTTGCGGGAGAGAACAACAGAGT4590.10303284705121787No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4590.10303284705121787No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT4550.10213495731656674No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4520.10146154001557839No Hit
GAAACTCTGTCAACGTATTGTGGGTCTTTTGGGGTTTGCTGCCCCTTTTA4460.10011470541360168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)