FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506661
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA35550.7016525842723241No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22640.4468471028952298No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT15650.30888503358261243No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT14850.29309538330362905No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT14470.28559529942111195No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12820.2530291457207087No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11990.23664738355626347No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT11370.22441040459005132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10870.2145418731656867No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10540.20802864242560606No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC9930.19598903408788124No Hit
TTCTTCTACTGTGCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC9340.184344167007131No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT9240.18237046072225807No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8990.17743619501007576No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8910.17585722998217743No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT8810.1738835236973045No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8720.17210718804091887No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8490.16756766358571115No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT8060.15908072656075758No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTAATTTTTCGT8050.1588833559322703No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7860.15513331399101174No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7840.15473857273403716No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7690.15177801330672777No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7560.14921219513639297No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7550.14901482450790568No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7530.1486200832509311No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGCAGCA6930.13677784554169356No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6800.13421202737135876No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6750.1332251742289223No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTA6730.13283043297194772No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6730.13283043297194772No Hit
ATGTCTCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACCTG6600.13026461480161292No Hit
ATCTAGAAGATCCCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6540.12908039103068916No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG6380.12592246097489246No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC6370.1257250903464052No Hit
CCATATACTTGGGAACAACATCTACAGCATGGGGCAGAATTTGTCCACCA6310.12454086657548143No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6270.12375138406153227No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6230.1229619015475831No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6200.12236978966212121No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6150.12138293651968476No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT5970.1178302652069135No Hit
ATCAACCATCTAGGGACCTAGTAGTCAATTATGTCAACACTAATATGGGC5960.1176328945784262No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA5920.11684341206447703No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA5900.11644867080750246No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5680.11210651698078203No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5680.11210651698078203No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT5620.11092229320985827No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTAATTTTTCGTGTGATGTGTT5560.10973806943893451No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5550.10954069881044723No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5530.10914595755347263No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA5440.10736962189708701No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA5320.10500117435523951No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5300.10460643309826492No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA5240.10342220932734116No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC5130.10125113241398094No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT5100.10065902052851906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)