FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506661
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA31800.6276385985895895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22540.44487339661035685No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16990.3353326977999096No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT14100.27829258616708213No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT12860.2538186282346579No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12790.25243703383524685No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11100.21908139762089446No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC10920.21552872630812317No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10580.20881812493955526No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT10040.19816011100124145No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9620.18987054460477518No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9360.1847389082641056No Hit
TTCTTCTACTGTGCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC9280.18315994323620724No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9150.18059412506587244No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT9130.18019938380889786No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT8950.1766467124961266No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8360.16500184541537635No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT8240.16263339787352885No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8190.16164654473109238No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8110.16006757970319405No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7740.15276486644916423No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7730.15256749582067694No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7580.14960693639336756No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7430.14664637696605817No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTA6770.13361991548589688No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTAATTTTTCGT6760.1334225448574096No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA6730.13283043297194772No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6650.13125146794404938No Hit
ATGTCTCCCTCCTGTTGCTGTACCAAACCTTCGGCCGGAAATTGCACCTG6560.12947513228766375No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6270.12375138406153227No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6220.12276453091909581No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA6060.11960660086329912No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6000.11842237709237538No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT5900.11644867080750246No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG5860.11565918829355329No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC5780.11408022326565494No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCCGTGG5730.11309337012321849No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA5670.11190914635229472No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5640.11131703446683285No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG5620.11092229320985827No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5600.11052755195288368No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT5550.10954069881044723No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT5500.10855384566801077No Hit
CCATATACTTGGGAACAACATCTACAGCATGGGGCAGAATTTGTCCACCA5450.10756699252557429No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5370.10598802749767597No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5370.10598802749767597No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTAATTTTTCGTGTGATGTGTT5330.1051985449837268No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA5240.10342220932734116No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5230.10322483869885386No Hit
CTTCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTA5230.10322483869885386No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5190.10243535618490471No Hit
CTTCTACTGTGCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT5130.10125113241398094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)