FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527960
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26300.49814379877263426No Hit
ATTTAATGAGTAACCCCATCTTTTTGTTTTGTCAGGGTTTAAATGTATAC16760.31744829153723764No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC14480.2742632017577089No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTCAGGGTTTAAATGTATACCC12540.23751799378740812No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12520.23713917721039474No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGAAAAAACCTAGCAGG12250.2320251534207137No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGAAAAAAC12120.22956284567012655No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG12050.22823698765057956No Hit
CATTTACACCAAGACATTATCGAAAGATGTGAACAATTTGTAGGCCCACT11430.21649367376316386No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT11390.21573604060913706No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11080.20986438366542923No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG10600.20077278581710736No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9700.1837260398515039No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT9030.17103568452155465No Hit
CTCAAGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTC8490.1608076369421926No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7990.15133722251685736No Hit
CTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7960.15076899765133722No Hit
CTCTATGACCGTTTCTCTCCCAAAAGTGAGACAAGAAATGTGAAACCACA7770.1471702401697098No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT7710.1460337904386696No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7560.143192666111069No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7530.1426244412455489No Hit
CACTTACAGTTAATGAGAAGAGAAGACTGAAATTAATTATGCCTGCTAGG7370.13959390862944163No Hit
ATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGA7110.1346692931282673No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7040.13334343510872038No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6970.13201757708917342No Hit
ATATAGAGCTGTGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6940.1314493522236533No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6810.12898704447306614No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCAGCAAA6680.126524736722479No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6580.12463065383741194No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6500.12311538752935829No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA6230.11800136373967725No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTG6120.1159178725661035No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6040.11440260625804985No Hit
GCTCTATATCGGGACGCTTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6010.11383438139252974No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGTCTTTATTCTTCTACTG6010.11383438139252974No Hit
ATAAACTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTA5970.11307674823850293No Hit
CTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC5950.1126979316614895No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5810.11004621562239562No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5770.10928858246836881No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC5750.1089097658913554No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5710.10815213273732859No Hit
GATTTATTAGATCCCCCCAGCAAAGGATTGCTTGCCTGAGTGCAGTATGG5680.10758390787180847No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5680.10758390787180847No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGAAAAAA5570.10550041669823472No Hit
AGAGTGTACAGCATCTTGAGTCCCTTTTCACTGCTGTTACCAATTTTCTT5550.1051216001212213No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5530.10474278354420789No Hit
GTTTAATATGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAG5460.10341692552466096No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5460.10341692552466096No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5430.10284870065914084No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5410.10246988408212743No Hit
GTTTAATATGATCTTGTGGCAATGACCCATAACTTCCAATGACATAGCCC5410.10246988408212743No Hit
CTTACAGTTAATGAGAAGAGAAGACTGAAATTAATTATGCCTGCTAGGTT5380.10190165921660731No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA5380.10190165921660731No Hit
CTCCTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT5370.10171225092810061No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCATATTAAAC5350.10133343435108719No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGTCTTTATTC5280.10000757633154027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)