FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451987
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA22830.5051030228745517No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT18980.41992358187292994No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17340.3836393524592521No Hit
CCTCCAGCTTACAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA14660.3243456117100713No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12480.2761141360260361No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT11710.2590782478257118No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11700.2588570025244089No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11450.25332586999183604No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10870.24049364251626706No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTCTAAATTTCAT10550.23341379287457384No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10400.23009511335503013No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9550.21128926274428245No Hit
GATCTCGTACTGCAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCC9010.19934201647392513No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8610.1904922044218086No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8090.1789874487540571No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7960.1761112598371192No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC7680.16991639140063763No Hit
GATCCAGCATCTAGGGACCTAGTAGTTAGTTATGTCAACACTAATATGGG7660.1694739007980318No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7590.1679251836889114No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7480.16549148537457936No Hit
CCCAAGTAGCTAGAGTCATTAGATCCCCCCAGCAAAGGATTGCTTGCCTG7470.16527024007327645No Hit
GTCATAGAGTATTTGGTATCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7310.16173031525242984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG7290.161287824649824No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7170.15863288103418902No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7140.1579691451302803No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTGACCAATTTTCTT7050.15597793741855406No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6870.15199552199510163No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6790.15022555958467831No Hit
CTCCGAAAGATACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6710.14845559717425502No Hit
GTACGACATCTTCTTGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGA6540.1446944270521055No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6530.14447318175080257No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6450.14270321934037924No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC6410.14181823813516758No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6380.14115450223125886No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6230.13783582271171516No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6200.1371720868078064No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6130.13562336969868602No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6050.13385340728826273No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6020.13318967138435397No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5950.1316409542752336No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5920.13097721837132484No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5890.13031348246741611No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5840.12920725596090155No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5670.125446085838752No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5610.12411861403093451No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5580.12345487812702577No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAAA5450.1205786892100879No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5440.12035744390878499No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5440.12035744390878499No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA5430.12013619860748208No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5410.11969370800487625No Hit
GGCCTGGTGTCCTTGTTGGGGTTGAAGTCCCCATCTGGATTTGCGGTGTT5370.11880872679966459No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5270.11659627378663547No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCTATGA5270.11659627378663547No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5200.11504755667751507No Hit
TTAGTTCCCCCCAGCAAAGGATTGCTTGCCTGAGTGCAGTATGATGAGGT5200.11504755667751507No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5170.11438382077360633No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5160.11416257547230341No Hit
ACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTG5040.11150763185666845No Hit
CACCTGTCCAGACGCCAACAAGGTAGGAGCAAATCCGCCTCCTGCATCTA5030.11128638655536555No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5020.11106514125406262No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4960.10973766944624512No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4860.107525216433216No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4860.107525216433216No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4840.10708272583061017No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG4810.10641898992670143No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4800.10619774462539851No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4740.10487027281758103No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4730.10464902751627812No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4660.10310031040715772No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4580.10133034799673442No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA4560.10088785739412859No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4560.10088785739412859No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4550.10066661209282568No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG4550.10066661209282568No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4520.10000287618891694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)