FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451987
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24370.5391747992752004No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT18590.41129501512211636No Hit
CCTCCAGCTTACAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA17420.38540931486967545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17230.3812056541449201No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12320.27257421120518954No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12180.2694767769869487No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTCTAAATTTCAT11820.26151194614004386No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11660.2579720213191973No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT10700.23673247239411754No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10410.23031635865633304No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9940.21991782949509608No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9750.21571416877034072No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC9220.2039881678012863No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8880.19646582755698727No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8350.18473982658793284No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7660.1694739007980318No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC7620.16858891959282016No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG7380.16327903236155022No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7330.16217280585503566No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7320.16195156055373275No Hit
GATCTCGTACTGCAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCC7220.15973910754070358No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7210.15951786223940068No Hit
GATCCAGCATCTAGGGACCTAGTAGTTAGTTATGTCAACACTAATATGGG7210.15951786223940068No Hit
GTCATAGAGTATTTGGTATCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7060.15619918271985697No Hit
CTCCGAAAGATACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA6930.1533229938029191No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6930.1533229938029191No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6850.1515530313924958No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTGACCAATTTTCTT6820.15088929548858707No Hit
CCCAAGTAGCTAGAGTCATTAGATCCCCCCAGCAAAGGATTGCTTGCCTG6800.15044680488598125No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA6700.1482343518729521No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6600.14602189885992298No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6570.14535816295601423No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6490.1435882005455909No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6440.14248197403907634No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6440.14248197403907634No Hit
GTACGACATCTTCTTGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGA6400.14159699283386468No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6350.1404907663273501No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT6270.13872080391692682No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT6250.13827831331432097No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6200.1371720868078064No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6180.1367295962052006No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5890.13031348246741611No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA5850.12942850126220445No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5730.12677355764656947No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5590.12367612342832869No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAAA5580.12345487812702577No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCTATGA5470.12102117981269372No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5470.12102117981269372No Hit
GGCCTGGTGTCCTTGTTGGGGTTGAAGTCCCCATCTGGATTTGCGGTGTT5450.1205786892100879No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5380.11902997210096751No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5350.11836623619705877No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5310.11748125499184711No Hit
ACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTG5160.11416257547230341No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5110.11305634896578885No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5110.11305634896578885No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5100.11283510366448593No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5090.11261385836318302No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTG5080.1123926130618801No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5070.11217136776057719No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4980.11018016004885095No Hit
CACCTGTCCAGACGCCAACAAGGTAGGAGCAAATCCGCCTCCTGCATCTA4960.10973766944624512No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4880.10796770703582181No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4860.107525216433216No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4780.10575525402279269No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA4730.10464902751627812No Hit
TTAGTTCCCCCCAGCAAAGGATTGCTTGCCTGAGTGCAGTATGATGAGGT4660.10310031040715772No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4660.10310031040715772No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4650.10287906510585482No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4650.10287906510585482No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT4640.1026578198045519No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4550.10066661209282568No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4540.10044536679152277No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4530.10022412149021985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)