FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402212
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA33250.8266784680715643No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT23500.5842689924716319No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT14740.3664734020864619No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13510.33589251439539347No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13380.33266038805406106No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC13000.32321263413324314No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT12610.31351625510924586No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12590.31301900490288703No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT12230.304068501188428No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11090.2757252394259744No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10710.2662774855051565No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10280.2555866060684415No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT10000.2486251031794178No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC9870.2453929768380854No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG9790.24340397601265007No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9790.24340397601265007No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT9470.2354479727109087No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9320.23171859616321738No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8890.22102771672650243No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT8840.21978459121060534No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA8440.20983958708342865No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA8360.2078505862579933No Hit
CACTAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT8140.20238083398804613No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGGCAAGAAATGTGAAACCACA8130.20213220888486672No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG8030.1996459578530725No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC7970.19815420723399602No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA7620.18945232862271638No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC7530.18721470269410162No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7460.18547432697184568No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7450.18522570186866627No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA7250.18025319980507792No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA7000.17403757222559246No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6970.1732916969160542No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6950.1727944467096954No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6890.17130269609061888No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6830.16981094547154238No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC6630.16483844340795403No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6500.16160631706662157No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6450.1603631915507245No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6420.15961731624118625No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6400.1591200660348274No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGCACG6200.15414756397123905No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA6160.15315306355852137No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6000.14917506190765067No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5990.14892643680447126No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5970.14842918659811244No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5910.1469374359790359No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5890.14644018577267712No Hit
CTCTTTTCCTAATATACATTTACACCAAGACATCATCAAAAAATGTGAAC5870.14594293556631827No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5820.14469981005042115No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5790.14395393474088292No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5770.1434566845345241No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5620.1397273079868328No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5590.13898143267729457No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5540.13773830716139748No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5520.13724105695503863No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC5490.13649518164550037No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5490.13649518164550037No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5430.13500343102642387No Hit
CTAATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTA5430.13500343102642387No Hit
ATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGACTCTA5250.13052817916919435No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5170.128539178343759No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5140.12779330303422076No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5100.1267988026215031No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT5090.12655017751832368No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5060.1258043022087854No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5040.1253070520024266No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG4940.1228208009706324No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTAC4880.1213290503515559No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4860.12083180014519707No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4840.12033454993883821No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4810.11958867462929998No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTTGATCTTC4780.11884279931976172No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4750.11809692401022345No Hit
TCCCGATACAGAGCTGAGGCGGTATCAAGAAGATCCCGTGCTGAAGGAAA4740.11784829890704404No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4740.11784829890704404No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATTAATTG4710.11710242359750579No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4680.11635654828796753No Hit
GGGTAGGTGGTAATTTGGAAGATCAAGCATCTAGGGACCTAGTAGTCAGT4670.11610792318478812No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4660.11585929808160869No Hit
GACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAG4660.11585929808160869No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4640.11536204787524987No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4610.11461617256571162No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4580.11387029725617336No Hit
ATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTACAAATTG4570.11362167215299396No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4550.1131244219466351No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4540.1128757968434557No Hit
CATTGGAAAACACCCTCTTTTCCTAATATACATTTACACCAAGACATCAT4520.11237854663709686No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCAT4520.11237854663709686No Hit
CCTAATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGT4500.111881296430738No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4490.11163267132755861No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4480.1113840462243792No Hit
GGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAA4430.11014092070848208No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4420.10989229560530267No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4410.10964367050212324No Hit
CCTAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTATTAGACG4370.10864917008940558No Hit
ATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGT4320.10740604457350851No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT4300.10690879436714967No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT4300.10690879436714967No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4220.10491979354171432No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4210.1046711684385349No Hit
ATACAGAGCTGAGGCGGTATCAAGAAGATCCCGTGCTGAAGGAAAGAAGT4180.10392529312899665No Hit
GGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAA4160.10342804292263783No Hit
GTGCAGTATGATGCGGTGAACAATGCTCAGGAGATTCTAAGGCTTCCCGA4120.10243354250992014No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA4110.10218491740674072No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4110.10218491740674072No Hit
AACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAAT4110.10218491740674072No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4080.10143904209720246No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4080.10143904209720246No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4060.10094179189084364No Hit
GTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGTATGATGTGAT4050.10069316678766423No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGG4040.1004445416844848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)