FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402212
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32680.8125068371903373No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT18850.4686583194932026No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC17250.4288783029844957No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG16470.4094855449365012No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13880.34509164321303193No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT13270.3299255119190874No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT13160.32719063578411384No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12550.3120245044901694No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11620.2889023698944835No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11380.2829353674181775No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT11030.27423348880689785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG10070.25036547890167377No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9990.2483764780762384No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9980.24812785297305898No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8860.22028184141696416No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGGCAAGAAATGTGAAACCACA8790.21854146569470828No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA8590.2135689636311199No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8590.2135689636311199No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8580.2133203385279405No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA8500.21133133770250512No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTCTGT8400.20884508667071097No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8200.2038725846071226No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7910.1966624566149195No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7720.19193857965451055No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA7690.19119270434497232No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA7680.19094407924179288No Hit
AATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG7670.19069545413861347No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT7320.18199357552733383No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT7290.1812477002177956No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7190.17876144918600143No Hit
CACTAATATGGGCCTAAAGTTCAGGCAAATATTGTGGTTTCACATTTCTT7050.17528069774148955No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA6780.1685678199556453No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA6630.16483844340795403No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC6600.16409256809841577No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6530.16235219237615983No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGCACG6460.16061181665390392No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC6440.16011456644754507No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6400.1591200660348274No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6390.15887144093164798No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6390.15887144093164798No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6220.1546448141775979No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6090.15141268783626544No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6000.14917506190765067No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5980.14867781170129185No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5710.1419649339154476No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC5690.14146768370908874No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5520.13724105695503863No Hit
CTCTTTTCCTAATATACATTTACACCAAGACATCATCAAAAAATGTGAAC5420.13475480592324446No Hit
TTCTAAAACAGTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGAC5370.13351168040734737No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5280.1312740544787326No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5250.13052817916919435No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5240.13027955406601494No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5200.12928505365329726No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATTAATTG5170.128539178343759No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5160.12829055324057959No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTAC5140.12779330303422076No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT5080.12630155241514424No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5060.1258043022087854No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC4940.1228208009706324No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4940.1228208009706324No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4940.1228208009706324No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT4920.12232355076427356No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4870.12108042524837648No Hit
ATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTACAAATTG4860.12083180014519707No Hit
GCTTTAGGGCATGGACCTTGACCCTTATAAAGAATTTGGAGCTACTGTGG4850.12058317504201764No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGG4850.12058317504201764No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4840.12033454993883821No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT4830.1200859248356588No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4780.11884279931976172No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTTGATCTTC4770.1185941742165823No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4740.11784829890704404No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4730.11759967380386464No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4710.11710242359750579No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4670.11610792318478812No Hit
ATACTGCACTCAGGCAAGCAATCCTTTGCTGGGGGGAGCTAATGACTCTA4670.11610792318478812No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4630.11511342277207046No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4630.11511342277207046No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAATGTATATT4530.11262717174027627No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4520.11237854663709686No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4510.11212992153391743No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC4500.111881296430738No Hit
GTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAATGACCCATAACATC4430.11014092070848208No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4410.10964367050212324No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4370.10864917008940558No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC4340.10790329477986732No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4320.10740604457350851No Hit
CTAATATACATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTA4290.10666016926397025No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4190.10417391823217606No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4190.10417391823217606No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG4180.10392529312899665No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4170.10367666802581722No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4160.10342804292263783No Hit
CATTGGAAAACACCCTCTTTTCCTAATATACATTTACACCAAGACATCAT4160.10342804292263783No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGACTGT4160.10342804292263783No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4160.10342804292263783No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4130.10268216761309956No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4120.10243354250992014No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4110.10218491740674072No Hit
AACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAAT4100.1019362923035613No Hit
GGGTAGGTGGTAATTTGGAAGATCAAGCATCTAGGGACCTAGTAGTCAGT4080.10143904209720246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)