FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582618
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28780.4939771857374815No Hit
GCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACCAGA23390.4014637378179185No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15410.2644957759629809No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACT9530.1635720145961848No Hit
TTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC9160.15722136974827416No Hit
GGATAAGGGTATTAAACCTTATTATCCGGAACATCTGGTTAATCATTACT8050.13816943520454225No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTT7660.13147551225674456No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC7470.1282143703078175No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC7110.12203536450985036No Hit
AGAATGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7010.12031897401041507No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGT6850.11757274921131856No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT6820.11705783206148797No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACCAGATGTTCCGGAT6740.11568471966193973No Hit
CCCTTATCCAATGGTAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6180.10607293286510201No Hit
GTATTAAACCTTATTATCCGGAACATCTGGTTAATCATTACTTCCAAACC6130.10521473761538436No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6100.10469982046555376No Hit
TCTCTGGAGTTACTCTCGTTTTTGCCTGGTGACTTCTTTCCTTCAGTACG5840.10023720516702196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)