FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385381
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24160.6269120688357754No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC17990.4668107664882285No Hit
CTCTATGGAAGGCGGGTATACTATATAAGAGAGAAACAACACATAGCGCC12280.3186457038618925No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACT12050.31267758400128703No Hit
CTTATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT11210.29088097233646704No Hit
CTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10690.2773878317820546No Hit
CTCTTATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9840.2553317366450344No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT9490.24624981511802607No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9320.241838596090622No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGATCACCATATT9050.23483254234121556No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8980.2330161580358139No Hit
CTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGATCACCATA8740.2267885547030082No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8290.21511179845399747No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA8060.20914367859339197No Hit
CCCTTATCCAATGGTAAATATTTGGTAACTTTTGGATAAAACCTAGCAGG7520.19513157109457913No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7230.1876065504007722No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC7200.1868280999841715No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACTTTTGGATAAAAC7120.1847522322065696No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACATC7000.18163843054016673No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT6960.1806004966513658No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6950.18034101317916557No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6920.17956256276256485No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6890.1787841123459641No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAATCCC6860.1780056619293634No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6780.17592979415176152No Hit
CTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTC6730.17463237679076032No Hit
GGTAAATATTTGGTAACTTTTGGATAAAACCTAGCAGGCATAATCAATTG6650.17255650901315842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6530.16944270734675554No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA6040.15672801720894386No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACTCACAGT6000.15569008332014292No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5840.1515383477649391No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5680.14738661220973528No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5670.14712712873753506No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAACCCTTGTATGCATGTATT5620.14582971137653386No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGCAATTGCACC5560.14427281054333246No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT5450.14141849234912982No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACTTT5350.13882365762712742No Hit
CTTCCGAGCAAACACTGCAAATCCAGATTGGGACTTCAATCCCAACAAGG5340.1385641741549272No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5330.13830469068272697No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAAT5240.13596933943292483No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT5240.13596933943292483No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5230.1357098559607246No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5200.13493140554412386No Hit
TAATAAATCTATCCACCTGGGTGGGTGGTAATTTGCAAGATCCAACATCC5040.13077966998892007No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4990.12948225262791885No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4910.12740638485031697No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4790.12429258318391409No Hit
CCTTATGAGTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4700.12195723193411197No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4610.11962188068430982No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4570.11858394679550886No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGCAATTGC4560.11832446332330862No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4540.11780549637890815No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4510.11702704596230744No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4480.11624859554570671No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4470.11598911207350648No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4430.11495117818470552No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4410.11443221124030506No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC4370.1133942773515041No Hit
GATTTATTAGGTCCCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATGG4360.11313479387930385No Hit
CTACAAACTTTGCCAACAAATCCGCCTCCTGCCTCGACCAATCGCCAGTC4320.11209685999050291No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4300.11157789304610242No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4300.11157789304610242No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4290.11131840957390218No Hit
GCTCTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAATCCCAGAGGATT4260.11053995915730147No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4220.10950202526850053No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4220.10950202526850053No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4200.10898305832410003No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4160.10794512443529909No Hit
GTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGAC4160.10794512443529909No Hit
GCCTGAGGATGCGTGTTTCTCAGAGGTGGAGACAGAGGGGTAGGCTGCCT4160.10794512443529909No Hit
GTAAAAGCCTGCTTAGATTGAATACATGCATACAAGGGTTTTAAAGCAGG4070.10560977318549694No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4060.10535028971329671No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4050.10509080624109647No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3990.10353390540789505No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3970.10301493846349456No Hit
GAATACATGCATACAAGGGTTTTAAAGCAGGATAACCACATTGTGTAAAA3960.10275545499129432No Hit
GTTATGTTAACACTAACATGGGACTAAAATTCAGGCAACTATTGTGGTTT3940.10223648804689384No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT3930.10197700457469361No Hit
TGCAAGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGA3930.10197700457469361No Hit
GTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA3930.10197700457469361No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG3920.10171752110249338No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3860.10016062026929194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)