FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385381
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24060.624317234113773No Hit
CTTATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12260.31812673691749205No Hit
CTCTATGGAAGGCGGGTATACTATATAAGAGAGAAACAACACATAGCGCC12170.31579138566768994No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC12010.31163965011248607No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACT11580.30048186080787587No Hit
CTCTTATATAGTATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10280.2667490094218449No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10200.264673141644243No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACTTTTGGATAAAAC9350.24261704650722274No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9290.2410601456740213No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9040.23457305886901533No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGATCACCATATT8980.2330161580358139No Hit
CTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8960.23249719109141342No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATACGTTGACAGACT8330.2161497323427984No Hit
CTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGATCACCATA8140.21121954637099388No Hit
CTTTAGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA7910.2052514265103884No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7900.20499194303818818No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT7780.20187814137178534No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACATC7710.20006175706638366No Hit
CCCTTATCCAATGGTAAATATTTGGTAACTTTTGGATAAAACCTAGCAGG7700.19980227359418343No Hit
GGTAAATATTTGGTAACTTTTGGATAAAACCTAGCAGGCATAATCAATTG7380.1914988024837758No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA6990.1813789470679665No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAATCCC6950.18034101317916557No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6920.17956256276256485No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACTTT6760.17541082720736104No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6560.17022115776335625No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6540.16970219081895577No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6340.164512521374951No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6340.164512521374951No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6320.16399355443055055No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6210.1611392362363479No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAACCCTTGTATGCATGTATT6190.16062026929194745No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC5450.14141849234912982No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGCAATTGCACC5440.1411590088769296No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT5420.1406400419325291No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5390.1398615915159284No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5320.1380452072105267No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5310.13778572373832648No Hit
TAATAAATCTATCCACCTGGGTGGGTGGTAATTTGCAAGATCCAACATCC5150.13363398818312267No Hit
CTTCCGAGCAAACACTGCAAATCCAGATTGGGACTTCAATCCCAACAAGG5150.13363398818312267No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT5100.1323365708221215No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5050.1310391534611203No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACTCACAGT5010.13000121957231933No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5000.1297417361001191No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG4960.12870380221131814No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT4860.12610896748931577No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4800.12455206665611435No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4780.12403309971171386No Hit
CTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTC4570.11858394679550886No Hit
CCTTATGAGTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4520.11728652943450767No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4490.11650807901790695No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGCAATTGC4490.11650807901790695No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4480.11624859554570671No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4480.11624859554570671No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4470.11598911207350648No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4460.11572962860130623No Hit
GAATACATGCATACAAGGGTTTTAAAGCAGGATAACCACATTGTGTAAAA4400.1141727277681048No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4350.11287531040710362No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTA4310.11183737651830267No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4170.10820460790749932No Hit
GATTTATTAGGTCCCCCCAGCAAAGAATTGCTTGCCTGAGTGCAGTATGG4160.10794512443529909No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4130.10716667401869838No Hit
GGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACTTTTGGATAAAA4130.10716667401869838No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4100.10638822360209765No Hit
GCTTTAAAACCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTTACTTT4090.10612874012989743No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4070.10560977318549694No Hit
GCTCTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAATCCCAGAGGATT4070.10560977318549694No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4050.10509080624109647No Hit
GTCCAGGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGAC4050.10509080624109647No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4020.10431235582449576No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4020.10431235582449576No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAACATGGGACTAAAATTCAGG3990.10353390540789505No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAAT3940.10223648804689384No Hit
GTTATGTTAACACTAACATGGGACTAAAATTCAGGCAACTATTGTGGTTT3920.10171752110249338No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT3920.10171752110249338No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3910.10145803763029315No Hit
CTACAAACTTTGCCAACAAATCCGCCTCCTGCCTCGACCAATCGCCAGTC3910.10145803763029315No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC3910.10145803763029315No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3880.10067958721369243No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC3870.10042010374149218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)