FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350632
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG19400.5532866367017272No Hit
ATTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA13830.3944306281229323No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT13050.37218508293595565No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12940.3690478906659974No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12870.3670514955851149No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA11710.3339683771019188No Hit
ACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA11710.3339683771019188No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG9250.26380934997376165No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT9100.2595313605147277No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9000.25667936754203835No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8890.2535421752720801No Hit
CTCTAGAACAGTTTCTCTTCCAAACATAAGACAGGAAACGTGAAACCACA7940.22644824203153163No Hit
TTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAA7850.22388144835611126No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAACATAAGA7810.22274065116703554No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT7810.22274065116703554No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG7610.21703666522165688No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7590.21646626662711904No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT7570.21589586803258115No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7410.21133267927627825No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7280.20762508841178215No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAATTATT7260.20705468981724431No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7150.20391749754728605No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6910.19707271441283167No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6790.19365032284560452No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG6510.18566474252207443No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6440.1836683474411919No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA6310.1799607565766958No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6230.17767916219854435No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTAATAATATCCTCTTTC6190.17653836500946862No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5660.16142280225421524No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5500.15685961349791233No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5480.15628921490337447No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5480.15628921490337447No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5400.15400762052522302No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5300.15115562755253373No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTGAAA5240.14944443176892014No Hit
GTTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5200.1483036345798444No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5190.14801843528257547No Hit
GCAATAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTAGCCAC5190.14801843528257547No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA5140.1465924387962308No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4840.13803645987816288No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4750.13546966620274248No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4750.13546966620274248No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4700.13404366971639783No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4660.13290287252732208No Hit
ATGTTAACATGGGCCTAAAATTCAGACAATTATTGTGGTTTCACGTTTCC4660.13290287252732208No Hit
GGGAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGA4610.13147687604097744No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCAAGGTGGTCTCCATGC4600.1311916767437085No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4600.1311916767437085No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4530.12919528166282598No Hit
CCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAACATAAGACA4530.12919528166282598No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAG4510.12862488306828812No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4460.12719888658194345No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT4450.12691368728467453No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC4450.12691368728467453No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTGTTCG4420.12605808939286772No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT4400.1254876907983299No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4370.12463209290652308No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4340.1237764950147163No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4320.12320609642017842No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT4270.12178009993383376No Hit
TTACTGGGCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAA4270.12178009993383376No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4260.12149490063656483No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4240.12092450204202697No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA4230.12063930274475802No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCA4230.12063930274475802No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCAATAAGGCA4210.12006890415022016No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACA4150.1183577083666066No Hit
ACATAAGACAGGAAACGTGAAACCACAATAATTGTCTGAATTTTAGGCCC4080.11636131328572406No Hit
GTGTACTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATAAGTTTTGC4080.11636131328572406No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCA4070.11607611398845513No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4040.11522051609664834No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA4010.11436491820484154No Hit
CATCCATTTGAAAGAGGATATTATTAACAGATGTCAACAATTTGTAGGCC3990.11379451961030368No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3970.11322412101576582No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3970.11322412101576582No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3960.11293892171849687No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC3950.11265372242122795No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA3930.11208332382669009No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG3910.11151292523215223No Hit
ACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGT3890.11094252663761436No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC3790.10809053366492505No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3790.10809053366492505No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA3750.10694973647584931No Hit
TATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA3740.10666453717858039No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3730.10637933788131144No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3720.10609413858404253No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA3700.10552373998950466No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAA3690.10523854069223575No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3650.10409774350316002No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3620.10324214561135321No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3590.10238654771954642No Hit
GAATATGGTGACCCAAAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA3510.10010495334139496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)