FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350632
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG17470.49824317232882337No Hit
ATTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA13570.3870154463939401No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12260.34965433845171007No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA12210.3482283419653654No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10950.31229323050948005No Hit
ACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA10900.3108672340231354No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10770.30715964315863925No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG10660.304022450888681No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9260.2640945492710306No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT8610.24555659494855003No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8510.24270460197586075No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG8170.23300782586871704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG8120.2315818293823724No Hit
TTATTAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAA7970.22730383992333844No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7620.21732186451892582No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT7600.21675146592438796No Hit
CTCTAGAACAGTTTCTCTTCCAAACATAAGACAGGAAACGTGAAACCACA7560.21561066873531223No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7470.21304387505989186No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACAATTATT7160.20420269684455497No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7140.2036322982500171No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC6980.1990691094937142No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAACATAAGA6960.19849871089917637No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6900.19678751511556275No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6580.18766113760295694No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTAATAATATCCTCTTTC6540.1865203404138812No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6520.18594994181934335No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6450.18395354673846084No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT6450.18395354673846084No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT6120.1745419699285861No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTGAAA6010.17140477765862785No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5590.1594264071733327No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5520.1574300120924502No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5390.1537224212279541No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA5390.1537224212279541No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5170.1474480366880376No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5130.14630723949896188No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA5050.14402564512081042No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5040.14374044582354148No Hit
GTTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5000.14259964863446575No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA5000.14259964863446575No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4960.14145885144539003No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAG4960.14145885144539003No Hit
GCAATAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTAGCCAC4900.13974765566177644No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTATCGTCCCCTTCTTCGTCTGCCG4810.13718086198635607No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4760.13575486550001142No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4720.1346140683109357No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4470.1274840858792124No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4460.12719888658194345No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4450.12691368728467453No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4430.12634328869013667No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA4430.12634328869013667No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCAATAAGGCA4410.1257728900955988No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4390.12520249150106094No Hit
ATGTTAACATGGGCCTAAAATTCAGACAATTATTGTGGTTTCACGTTTCC4320.12320609642017842No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT4210.12006890415022016No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4200.11978370485295124No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4190.1194985055556823No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT4160.11864290766387552No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4150.1183577083666066No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4130.11778730977206874No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC4130.11778730977206874No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4100.11693171188026193No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4080.11636131328572406No Hit
CCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAACATAAGACA4070.11607611398845513No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC4050.11550571539391727No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG4050.11550571539391727No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA4000.1140797189075726No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3980.11350932031303473No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC3970.11322412101576582No Hit
GGGAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGA3960.11293892171849687No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA3960.11293892171849687No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT3940.11236852312395904No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT3930.11208332382669009No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3930.11208332382669009No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCATGTTCA3920.11179812452942117No Hit
GTGTACTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATAAGTTTTGC3850.10980172944853864No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTGTTCG3850.10980172944853864No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGCA3840.10951653015126972No Hit
CATCCATTTGAAAGAGGATATTATTAACAGATGTCAACAATTTGTAGGCC3840.10951653015126972No Hit
ACATAAGACAGGAAACGTGAAACCACAATAATTGTCTGAATTTTAGGCCC3820.10894613155673184No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC3810.10866093225946291No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAATTCAGACA3790.10809053366492505No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC3790.10809053366492505No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC3770.10752013507038718No Hit
ACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGT3750.10694973647584931No Hit
GTGTGAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT3700.10552373998950466No Hit
GAATATGGTGACCCAAAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA3680.1049533413949668No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3660.10438294280042894No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3630.10352734490862216No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3620.10324214561135321No Hit
CCCAAAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATATAAAATACCAG3590.10238654771954642No Hit
TTACTGGGCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAA3590.10238654771954642No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA3560.10153094982773962No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT3550.10124575053047069No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT3550.10124575053047069No Hit
CCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATATAAAATACCAG3540.10096055123320176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)