FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371637
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG21970.5911682636551259No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT17190.4625481316445887No Hit
GTATCTCTTGTTCCCAAGTATATGGTGACCCACAAAATGAGGCACTGCGT16680.448825063166477No Hit
GATTAAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT15370.41357561276191557No Hit
CTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12910.3473819883380826No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG12610.33930959511566394No Hit
GTAAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTA12300.33096812211916465No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA12300.33096812211916465No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11830.31832137273737543No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG11770.3167068940928917No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA11640.31320885702984363No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11220.3019075065184575No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT11010.2962568312627645No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG10640.28630087962178147No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9670.26020014153596116No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA9540.2567021044729131No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9360.25185866853946187No Hit
GTTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTACAAATTGTTGA9130.2456698337356076No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA8920.24001915847991456No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8860.2384046798354308No Hit
ACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACAT8840.23786652028726954No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8360.22495069113139973No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8100.21795461700530355No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7880.2120348619755299No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTATACCTGTC7820.21042038333104618No Hit
CATTTAAACCCTAATAAAACCAAACGGTGGGGTTACTCCCTTAACTTCAT7810.21015130355696554No Hit
CTTTTACACAGTGTGGTTACCCAGCCTTGATGCCTTTATATGCATGTATA7520.2023479901086275No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT7520.2023479901086275No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT7520.2023479901086275No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT7470.2010025912382244No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7450.20046443169006314No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7400.19911903281966004No Hit
GTATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA7280.1958900755306926No Hit
AGTATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTAT7230.1945446766602895No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC7230.1945446766602895No Hit
GTGTGGTTACCCAGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC7110.19131571937132202No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6850.18431964524522584No Hit
GTTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTACAAATTGTTGA6690.1800143688599359No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC6640.1786689699895328No Hit
TCCTCAGGCCGTGCAGTGGAACTCAAGCACCTTCCACCAAGCTCTGCTAG6580.17705449134504905No Hit
ACTCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6470.17409461383016223No Hit
GCTTTATTCTTCTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCT6360.1711347363152754No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6290.169251177896711No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6090.1638695824150986No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6070.16333142286693736No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTA5990.1611787846742924No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5700.15337547122595438No Hit
ATGTTAACATGGGCATAAAAATCAGACAATTATTGTGGTTTCACATTTCC5700.15337547122595438No Hit
TAACCACACTGTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCT5680.1528373116777931No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCACTCGGCAGT5620.15122283303330938No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5540.14907019484066442No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5420.14584123755169695No Hit
TTCTTCTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5370.14449583868129384No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTAA5270.14180504094048763No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA5240.14099780161824577No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5210.1401905622960039No Hit
ATACAGCACTAAGGCAAATTATTCTCTGTTGGGGTGAGTTGATGAATCTG5190.13965240274784266No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5100.13723068478111708No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5050.13588528591071394No Hit
ATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGC5030.1353471263625527No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5030.1353471263625527No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT5020.13507804658847208No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACGCCTCACCATACAG4900.13184908929950462No Hit
GATTGAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4880.1313109297513434No Hit
TCACCATACAGCACTAAGGCAAATTATTCTCTGTTGGGGTGAGTTGATGA4850.1305036904291015No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA4750.1278128926882953No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4750.1278128926882953No Hit
GTATACATGCATATAAAGGCATCAAGGCTGGGTAACCACACTGTGTAAAA4720.12700565336605343No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4700.12646749381789218No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT4700.12646749381789218No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTAG4680.12592933426973094No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG4660.1253911747215697No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC4660.1253911747215697No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4590.12350761630300536No Hit
ACCCAGCATCCAGGGAATCAGTAGTCAGCTATGTCAATGTTAACATGGGC4580.12323853652892473No Hit
ACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA4580.12323853652892473No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4510.12135497811036039No Hit
GCCTTAGAGTCTCCGGAACATTGTACGCCTCACCATACAGCACTAAGGCA4510.12135497811036039No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4500.12108589833627977No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA4440.11947141969179602No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG4410.11866418036955416No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT4390.11812602082139291No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC4380.11785694104731229No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA4320.11624246240282857No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4300.11570430285466732No Hit
ATGTTAGGAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTATAGT4240.1140898242101836No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT4210.11328258488794174No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT4210.11328258488794174No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4200.11301350511386112No Hit
GTAACCACACTGTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTC4200.11301350511386112No Hit
CTCTAAAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4190.11274442533978049No Hit
GTCCAAGGGATACTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC4160.11193718601753863No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGGTT4140.11139902646937737No Hit
GTTTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC4070.10951546805081303No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGG4070.10951546805081303No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4020.10817006918040992No Hit
GTAGTCAGCTATGTCAATGTTAACATGGGCATAAAAATCAGACAATTATT4000.10763190963224867No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTC3980.10709375008408743No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3970.10682467031000681No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3960.10655559053592618No Hit
ATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATTTACAT3940.10601743098776495No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTG3900.10494111189144247No Hit
GGATTAAAGACAGGTATAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3840.10332663324695873No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3820.10278847369879747No Hit
TCCTAACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG3790.10198123437655561No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3770.10144307482839438No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3760.10117399505431375No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3720.10009767595799127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)