FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371637
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG23950.6444460589230889No Hit
GTATCTCTTGTTCCCAAGTATATGGTGACCCACAAAATGAGGCACTGCGT17560.47250408328557164No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT15570.418957208243528No Hit
GATTAAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT13230.3559925411086625No Hit
CTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12240.32935364347468093No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT11960.3218194098004235No Hit
TCCTCACATTCATTTGAAAGAGGATATTATCAACAGATGTCAACAATTTG11560.3110562188371987No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCA11560.3110562188371987No Hit
ATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAA11410.30702002222598934No Hit
GGATATTATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG11360.3056746233555862No Hit
GTAAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTA11250.30271474584069935No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10850.29195155487747454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10830.2914133953293133No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGG10740.2889916773625877No Hit
GTTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTACAAATTGTTGA10310.27742124707712096No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9980.26854161453246045No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9590.2580475033433162No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA9200.24755339215417194No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA9120.24540075396152697No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8610.23167768548341527No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8130.2187618563275454No Hit
ACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACAT8070.2171473776830617No Hit
CTTTTACACAGTGTGGTTACCCAGCCTTGATGCCTTTATATGCATGTATA8060.21687829790898108No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7940.21364934062001362No Hit
AGTATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTAT7890.2123039417496105No Hit
GTATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA7850.21122762265328804No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT7830.2106894631051268No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC7740.2082677451384012No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7710.20746050581615932No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7600.20450062830127247No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGTTGATAATATCCTCTTTC7520.2023479901086275No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTATACCTGTC7250.19508283620845074No Hit
GTTTTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCTACAAATTGTTGA7190.193468357563967No Hit
CATTTAAACCCTAATAAAACCAAACGGTGGGGTTACTCCCTTAACTTCAT7180.19319927778988635No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTA7120.19158479914540263No Hit
GTGTGGTTACCCAGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC6960.1872795227601127No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT6910.18593412388970956No Hit
GGATAGAACCTAGCAGGCATAATCAGTTTTAATCTCCTTTTTTCATTAAC6860.18458872501930645No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6850.18431964524522584No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6620.17813081044137155No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT6430.17301829473383976No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6330.17032749699303354No Hit
ACTCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6260.16844393857446915No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6160.16575314083366297No Hit
TCCTCAGGCCGTGCAGTGGAACTCAAGCACCTTCCACCAAGCTCTGCTAG6080.163600502641018No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA5970.16064062512613114No Hit
GCTTTATTCTTCTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCT5930.15956430602980864No Hit
TTCTTCTACTATACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5800.15606626896676057No Hit
TAACCACACTGTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCT5690.15310639145187374No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5640.15176099258147063No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG5630.15149191280739No Hit
ATGTTAACATGGGCATAAAAATCAGACAATTATTGTGGTTTCACATTTCC5470.14718663642210006No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5460.14691755664801945No Hit
ATACAGCACTAAGGCAAATTATTCTCTGTTGGGGTGAGTTGATGAATCTG5180.13938332297376205No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5170.1391142431996814No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACGCCTCACCATACAG5150.13857608365152016No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT5140.13830700387743955No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5090.13696160500703644No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5030.1353471263625527No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5020.13507804658847208No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGGAT5020.13507804658847208No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4910.13211816907358526No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4910.13211816907358526No Hit
TATCAACAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATGAAAAAA4890.131580009525424No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4880.1313109297513434No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4780.12862013201053718No Hit
GTATACATGCATATAAAGGCATCAAGGCTGGGTAACCACACTGTGTAAAA4720.12700565336605343No Hit
GATTGAAACTGATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4680.12592933426973094No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTGTAA4660.1253911747215697No Hit
TCACCATACAGCACTAAGGCAAATTATTCTCTGTTGGGGTGAGTTGATGA4620.12431485562524722No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA4600.12377669607708597No Hit
GCGTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTA4570.12296945675484411No Hit
GCCTTAGAGTCTCCGGAACATTGTACGCCTCACCATACAGCACTAAGGCA4570.12296945675484411No Hit
GATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTAG4550.12243129720668287No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCACTCGGCAGT4490.12081681856219914No Hit
ACCCAGCATCCAGGGAATCAGTAGTCAGCTATGTCAATGTTAACATGGGC4460.12000957923995728No Hit
ATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG4420.11893326014363477No Hit
GTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4410.11866418036955416No Hit
CTCTAAAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4380.11785694104731229No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAG4380.11785694104731229No Hit
GTAGTCAGCTATGTCAATGTTAACATGGGCATAAAAATCAGACAATTATT4340.1167806219509898No Hit
GTTTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC4330.11651154217690919No Hit
ATGTTAGGAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTATAGT4290.11543522308058671No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4290.11543522308058671No Hit
GTAACCACACTGTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTC4280.11516614330650608No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTG4260.11462798375834483No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4220.11355166466202235No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAACTGATTATGCCTGCTAGGTT4200.11301350511386112No Hit
CAAGTATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTT4170.11220626579161926No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC4170.11220626579161926No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAG4130.11112994669529674No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC4120.11086086692121612No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4110.11059178714713551No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4050.10897730850265178No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGT4040.10870822872857115No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT3970.10682467031000681No Hit
GTGTAGTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAA3970.10682467031000681No Hit
ATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGC3970.10682467031000681No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACTCC3900.10494111189144247No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3820.10278847369879747No Hit
GTCTATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATA3810.10251939392471685No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC3780.10171215460247499No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT3750.10090491528023313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)