FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511472
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27840.5443113210498327No Hit
GTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGTTCAGG20450.399826383457941No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTCT17940.35075233834892233No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT17860.3491882253573998No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14860.2905339881753058No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14750.2883833328119623No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14370.2809537961022304No Hit
GTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA13770.2692229486658116No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12270.23989583007476462No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11380.22249507304407673No Hit
GTCTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10800.21115525385553852No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10460.20450777364156786No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT9940.19434103919667156No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT9470.18515187537147684No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9160.17909093752932712No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8950.17498514092658055No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8770.17146588669565488No Hit
GTTTGAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA8550.167164575968968No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8340.16305877936622143No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA8020.1568023274001314No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7900.1544561579128476No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7850.15347858729314606No Hit
CGTCCACAACGGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAG7680.15015484718616073No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT7560.14780867769887698No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7550.14761316357493665No Hit
ATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTA7510.14683110707917538No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7270.14213876810460788No Hit
GTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT7200.14077016923702568No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7190.14057465511308537No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7100.13881502799762255No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6990.1366643726342791No Hit
GAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGTTCA6940.13568680201457753No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6880.13451371727093564No Hit
TCCACAGAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCA6870.13431820314699533No Hit
TTGTCAACGTGCCCTGAGCCTGAGGGCTCCACCCCAACAGACCGCCGTGT6850.1339271748991147No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT6840.1337316607751744No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTGGGCGAGAAAGTGAAAGCCTGCT6690.1307989489160697No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6530.12767072293302467No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6390.1249335251978603No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6250.12219632746269592No Hit
CCCATCATCCAGGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGCC6040.11809053085994933No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG6030.117895016736009No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5890.11515781900084461No Hit
CGTTGACAACGGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAG5860.1145712766290237No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5780.11300716363750118No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5690.11124753652203836No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5680.11105202239809803No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5650.1104654800262771No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5520.10792379641505302No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5520.10792379641505302No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5490.10733725404323208No Hit
GTCCCGTGCTGGTTGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5460.10675071167141113No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5390.10538211280382895No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG5300.10362248568836613No Hit
TGTTTACACAGAAAGGCCTTGTAAGTGGGCGAGAAAGTGAAAGCCTGCTT5220.10205837269684362No Hit
TTGTGGACGTGCCCTGAGCCTGAGGGCTCCACCCCAACAGACCGCCGTGT5190.10147183032502267No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTGTACCTGTC5190.10147183032502267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)