FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511472
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27330.5343401007288766No Hit
GTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGTTCAGG21050.4115572308943598No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTCT15520.3034379203553665No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCTTAT14580.285059592704977No Hit
GTTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA13090.2559279882378703No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12960.25338630462664624No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12840.25104013513936246No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12150.23754966058748087No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11400.22288610129195732No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11130.21760721994556886No Hit
GTCTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10540.20607188663309037No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10330.2019660900303438No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9410.18397879062783495No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8850.1730299996871774No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT7840.15328307316920575No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC7690.15035036131010104No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT7620.14898176244251884No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7390.14448493759189165No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7370.144093909344011No Hit
GTTTGAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA7150.1397925986173241No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6960.13607783026245818No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6370.12454249694997965No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6360.12434698282603936No Hit
GTACTCAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT6340.12395595457815874No Hit
CGTCCACAACGGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAG6240.12200081333875558No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT6150.12024118622329276No Hit
GAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCATGTTCA6140.12004567209935246No Hit
ATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTA6060.11848155910782994No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6030.117895016736009No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5940.11613538962054618No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT5910.11554884724872526No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5860.1145712766290237No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.11300716363750118No Hit
TTGTCAACGTGCCCTGAGCCTGAGGGCTCCACCCCAACAGACCGCCGTGT5780.11300716363750118No Hit
GGATAATAAGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5700.11144305064597866No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5640.1102699659023368No Hit
CCCATCATCCAGGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGCC5600.10948790940657555No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTGGGCGAGAAAGTGAAAGCCTGCT5590.10929239528263521No Hit
TCCACAGAGTATGTAAATAATGCCTAGTCTTGAAGTAATGATTAACTGCA5570.10890136703475459No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5530.10811931053899333No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG5510.10772828229111273No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5360.104795570432008No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5290.1034269715644258No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG5230.10225388682078393No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5210.10186285857290331No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5170.10108080207714205No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5140.10049425970532111No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5130.1002987455813808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)