FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614928
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78441.2755964925975074No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT35100.5707985325111232No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA17710.28800119688809095No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT15270.2483217547420186No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14640.23807665287643429No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13790.22425389639112223No Hit
ATATGTTCCTGTGGTAATGTACCCCAACTCCCAATTACATATCCCATGAA12560.20425155465355294No Hit
CTTTTGTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12560.20425155465355294No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT12370.20116176202742436No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12330.20051127936929203No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12040.1957952800978326No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT11690.19010355683917465No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10530.171239559753337No Hit
GTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT9750.15855514791975645No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC9640.15676632060989254No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9570.15562797595816094No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG9390.15270080399656547No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9250.1504241146931023No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9190.14944839070590377No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8690.1413173574792496No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8670.14099211615018345No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8630.14034163349205112No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8310.13513777222699244No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT8280.13464991023339318No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8240.13399942757526084No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8160.13269846225899617No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7800.12684411833580517No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7310.11887570577368409No Hit
ACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACT7310.11887570577368409No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7280.11838784378008482No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT7270.11822522311555174No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7220.11741211979288632No Hit
GTCACCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTG7220.11741211979288632No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7120.11578591314755549No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7030.11432232716675773No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7020.11415970650222465No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6990.1136718445086254No Hit
GTTCAAATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6980.11350922384409232No Hit
GATCCAGGATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGG6910.11237087919236073No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6890.11204563786329456No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG6750.1097689485598314No Hit
TCCTTGATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAACT6670.10846798324356674No Hit
GTGGTAATGTACCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA6560.10667915593370282No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6420.10440246663023965No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6390.10391460463664039No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6320.10277625998490879No Hit
GGGTAAAGGTTCAAATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA6220.10115005333957797No Hit
ATGTAATTGGGAGTTGGGGTACATTACCACAGGAACATATTGTACAAAAA6210.10098743267504487No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6210.10098743267504487No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAG6190.1006621913459787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)