FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614928
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151692.4667928603023443No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT34400.5594150859938074No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA15900.2585668566076028No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT15090.2453945827804231No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13610.22132672442952672No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT12990.21124424322847551No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA12280.1996981760466266No Hit
ATATGTTCCTGTGGTAATGTACCCCAACTCCCAATTACATATCCCATGAA12030.19563265943329952No Hit
CTTTTGTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11780.19156714281997242No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10780.17530507636666406No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10770.175142455702131No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10590.17221528374053546No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10450.1699385944370723No Hit
GTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT10030.1631085265266828No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9680.15741680326802487No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC9530.15497749330002863No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT9410.15302604532563163No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9230.1500988733640361No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT9100.14798480472510603No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8650.14066687482111728No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8330.13546301355605858No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8310.13513777222699244No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7990.12993391096193374No Hit
GTTCAAATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7600.1235917050451435No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7580.12326646371607732No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7330.11920094710275023No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7280.11838784378008482No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7040.11448494783129082No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6920.11253349985689383No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6860.11155777586969531No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG6760.10993156922436448No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6750.1097689485598314No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6700.10895584523716596No Hit
GATCCAGGATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGG6670.10846798324356674No Hit
GTCACCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTG6640.10798012124996748No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6560.10667915593370282No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6540.10635391460463664No Hit
TCCTTGATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAACT6320.10277625998490879No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6250.10163791533317722No Hit
ATGTAATTGGGAGTTGGGGTACATTACCACAGGAACATATTGTACAAAAA6220.10115005333957797No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6220.10115005333957797No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6170.10033695001691256No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6170.10033695001691256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)