FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572951
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24690.43092690299868575No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCAGG22380.39060931912153046No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACC16200.28274669212550463No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT15150.2644205176358886No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14980.2614534227185222No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13900.24260364324348854No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG13030.22741909866637813No Hit
GTATAAAGGGAGTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC11460.20001710442952367No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11360.19827175447813167No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10810.18867232974547563No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10100.1762803450905924No Hit
CCCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTA9480.16545917539196195No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9370.16353929044543075No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA8920.15568521566416674No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT8470.14783114088290272No Hit
ATATAAGACTGCACCCCTATGACCATCTGATCTTCAACAAAGCTGACAAA8240.1438168359947011No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8080.14102427607247392No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8040.1403261360919171No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8030.1401516010967779No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8030.1401516010967779No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAC7900.13788264615996831No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC7780.1357882262182979No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7770.1356136912231587No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATA7600.13264659630579229No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7480.1305521763641219No Hit
GTGCTGGATATTAGACCTTTATTAGATGCATAGTTTGCAAGTATTTGCTC7360.1284577564224515No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7300.12741054645161626No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7270.12688694146619867No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7160.12496705651966747No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6650.11606577176756826No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT6650.11606577176756826No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6550.11432042181617626No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6460.11274960685992345No Hit
GAGTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCA6370.11117879190367065No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTACAA6360.11100425690853144No Hit
TCCTTACACCATATAAAAAATCAACTCAAGATGGACTAAAGGCTTAAATA6360.11100425690853144No Hit
CTCTTACAGCTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG6350.11082972191339224No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6240.10890983696686103No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT6140.10716448701546903No Hit
GTATAGGCCCCTCACTCTGGGATCCAGCAGAGCTTGGTGGAATGTGCTGG6070.10594274204949464No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5910.10315018212726743No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC5890.10280111213698902No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAACATTCATCTGAAAG5870.10245204214671064No Hit
CCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC5810.10140483217587543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)