FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464301
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64291.3846621049706977No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA20970.45164666886351745No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11660.2511301935597813No Hit
CATTTACACCAGGACATTATCAAAAAATGTGAACAGTTCGTAGGACCACT9280.19987034273025472No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT8530.18371702839321907No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT8440.18177863067277478No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTGGCAGG7970.17165588702156576No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7970.17165588702156576No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC7810.16820984662966482No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT7730.16648682643371435No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7660.16497918376225768No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT7350.1583024805029496No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7210.15528719516003628No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT7120.15334879743959198No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT6920.14904124694971582No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6870.14796435932724677No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6830.14710284922927153No Hit
TCGTAGGACCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG6470.1393492583474944No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTGGCAGGCATAATCAATTG6300.13568784043109966No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT6130.1320264225147049No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT6040.13008802479426063No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA5860.12621122935337206No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC5510.11867301599608875No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5480.11802688342260731No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT5340.115011598079694No Hit
GTTCGTAGGACCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA5170.11135018016329924No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5090.10962715996734877No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT4980.10725800719791688No Hit
GTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT4970.10704262967342307No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC4900.10553498700196638No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4670.10058130393860878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)