FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464301
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64201.3827237072502536No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA20850.4490621385695917No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12760.27482172125410026No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9090.19577816976487236No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT9030.1944859046179095No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8930.19233212937297142No Hit
CATTTACACCAGGACATTATCAAAAAATGTGAACAGTTCGTAGGACCACT8890.19147061927499617No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT8550.18414778344220667No Hit
GTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAAC8070.17380966226650385No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTCCTAAT7570.16304078604181338No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT7450.1604562557478877No Hit
CCCTTATCCAATGGTAAATATTTGGTAACCTTTGGATAAAACCTGGCAGG7370.15873323555193722No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7170.15442568506206103No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT7120.15334879743959198No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT7110.15313341991509818No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT6810.14667209418028393No Hit
ATATTTACCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6720.14473369645983963No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGTAAATATTTGGTAACCTT6590.14193378864142012No Hit
GGTAAATATTTGGTAACCTTTGGATAAAACCTGGCAGGCATAATCAATTG6380.13741086062705013No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6070.13073415736774205No Hit
TCGTAGGACCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG5840.12578047430438444No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT5620.12104216876552064No Hit
GTGTAAAAGGGGCAGCAAAACCTAAAAGACCCACAATTCGTTGACATACT5600.12061141371653301No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5510.11867301599608875No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCATCTTTTC5400.11630386322665684No Hit
ATCCAAAGGTTACCAAATATTTACCATTGGATAAGGGTATTAAACCTTAT5270.11350395540823734No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5200.11199631273678066No Hit
CTCTAAATTTTATGGGTTATGTCATTGGAAGTTATGGGTCCTTGCCACAA4790.10316583423253449No Hit
GTTCGTAGGACCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4730.10187356908557164No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGATTAAAGA4690.10101205898759641No Hit
CTTTAATCCTCATTGGAAAACACCATCTTTTCCTAATATACATTTACACC4650.10015054888962117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)