FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319574
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48431.5154549494013907No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9240.28913491085006915No Hit
CTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8770.27442783205141846No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT6320.19776327235632435No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG4830.1511387033988998No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4720.14769662112687515No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG4680.14644495484613892No Hit
CTCTTATATAGAATGCCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4550.1423770394337462No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC4320.13517995831951285No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4290.1342412086089607No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4250.13298954232822446No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4190.1311120429071201No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGTG4120.1289216269158317No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA4060.12704412749472735No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT3930.1229762120823346No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA3910.1223503789419665No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3850.12047287952086216No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC3840.12015996295067809No Hit
ATACAATATCATCCACATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3750.11734371381902159No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3740.11703079724883753No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC3520.11014663270478825No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC3450.10795621671349984No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAATCTCTCTTCCAAAAGTAAGG3400.10639163386257956No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA3360.10513996758184332No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT3300.10326246816073899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)