FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293021
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12900.4402414843987291No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA7750.26448616310776357No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6750.23035891625514895No Hit
ACTACACACTGTGCCAGCAAATCCACCTCCTGCCTCCACCAATCGCCAGT6320.21568420010852465No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6030.20578729852126643No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT5860.1999856665563219No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT5740.19589039693400814No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC5430.18531095040969758No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5400.18428713300411917No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5240.17882677350770082No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4820.16449332982960266No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC4790.16346951242402422No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC4700.1603980602072889No Hit
CACTAATATGGGCCTAAAGTTCAGACAGCTCTTGTGGTTTCACATTTCTT4650.15869169786465817No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA4570.15596151811644898No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4480.15289006589971368No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4420.1508424310885568No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT4240.14469952665508617No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4210.14367570924950773No Hit
GATTTAGATCTTCGGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4150.14162807443835085No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC4090.13958043962719396No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT4090.13958043962719396No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4050.13821534975308938No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT3960.13514389753635406No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT3930.13412008013077562No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT3850.13138990038256643No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG3770.12865972063435727No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3690.12592954088614808No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3650.1245644510120435No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT3650.1245644510120435No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3430.11705645670446826No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA3380.11535009436183755No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT3370.11500882189331141No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA3290.11227864214510222No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3270.11159609720804993No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3230.11023100733394535No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3180.10852464499131462No Hit
CCTCAGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCGCTTGGGACTC3160.10784210005426233No Hit
TGCTTGATCTTCCAAATTAACACCCACCCAGGTAGCCAGAGTCATTAATT3110.10613573771163158No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3100.10579446524310544No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAA3050.10408810290047472No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG3000.10238174055784398No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA2950.10067537821521326No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAGGCGCTATGT2950.10067537821521326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)