FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293021
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12810.4371700321819938No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA7450.2542479890519792No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7110.2426447251220902No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5850.19964439408779577No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT5790.1975967592766389No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT5730.195549124465482No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5640.19247767224874668No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5620.19179512731169437No Hit
ACTACACACTGTGCCAGCAAATCCACCTCCTGCCTCCACCAATCGCCAGT5400.18428713300411917No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5330.18189822572443612No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC4660.1590329703331843No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4430.15118370355708294No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4360.14879479627739992No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT4340.14811225134034764No Hit
GTGTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4170.14231061937540312No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA4080.13923916715866783No Hit
CACTAATATGGGCCTAAAGTTCAGACAGCTCTTGTGGTTTCACATTTCTT4070.13889789469014166No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4050.13821534975308938No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4020.13719153234751094No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC4000.13650898741045864No Hit
GATTTAGATCTTCGGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA3990.1361677149419325No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3960.13514389753635406No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT3930.13412008013077562No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3800.1296835380399357No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC3690.12592954088614808No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA3670.1252469959490958No Hit
CCTCAGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCGCTTGGGACTC3620.12354063360646506No Hit
CAAGATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGAT3560.12149299879530817No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA3500.11944536398415131No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC3500.11944536398415131No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3390.11569136683036368No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT3330.11364373201920681No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT3310.11296118708215452No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT3210.10954846239689306No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3200.10920718992836691No Hit
CATTTACACCAAGACATTGTCAAAAAATGTGAACAATTTGTAGGCCCACT3130.10681828264868388No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA3100.10579446524310544No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG3070.104770647837527No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3070.104770647837527No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT3060.10442937536900086No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT3040.10374683043194856No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT2960.1010166506837394No Hit
CTATTTACACACTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAA2960.1010166506837394No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA2950.10067537821521326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)