FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445062
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14870.33411075310855565No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14050.31568635381137905No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12040.2705241067536658No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11090.2491787661044978No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10140.2278334254553298No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9900.22244091834396107No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9660.21704841123259233No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9470.2127793431027587No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9270.20828558717661808No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9190.20648808480616182No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8180.18379461737915168No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8150.18312055399023058No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7950.17862679806408993No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7910.17772804687886182No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT7840.17615523230471258No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7690.17278491536010715No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7580.17031334960072977No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7510.16874053502658057No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7460.1676170960450454No Hit
CCCAAAACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGC7430.1669430326561243No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7390.16604428147089617No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7350.16514553028566809No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7340.16492084248936104No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7020.15773083300753601No Hit
CCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGC6860.15413582826662353No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGCAGTAA6830.15346176487770244No Hit
GGTTCTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTG6680.15009144793309695No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6530.1467211309884915No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6450.14492362861803523No Hit
CATCCAACGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC6360.14290143845127196No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6180.1388570581177454No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGAACCACATC6090.13683486795098213No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5980.13436330219160478No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5980.13436330219160478No Hit
GTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5960.1339139265989907No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGCAGT5940.13346455100637664No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5860.13166704863592038No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT5850.13144236083961336No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5810.13054360965438522No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5690.12784735609870085No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5690.12784735609870085No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5630.12649922932085866No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5590.12560047813563055No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5530.12425235135778835No Hit
CTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5390.12110672220948993No Hit
ATAAAATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAG5390.12110672220948993No Hit
CTTCCAGGGGACCTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGT5340.11998328322795476No Hit
GTATGGATCGGCAGAGGAGACACGAAGGTTCCACGCATGCGCTGATGGCC5270.11841046865380554No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5090.11436608832027896No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG4920.11054639578305944No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCAAGGAGATCTCGAA4910.11032170798675242No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4860.10919826900521726No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4790.10762545443106802No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4750.10672670324583991No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTCTGGGGGC4690.10537857646799771No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4680.10515388867169068No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4670.10492920087538365No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4660.10470451307907662No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4650.10447982528276958No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTC4630.10403044969015551No Hit
CCTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTGCCCACCCAGGT4610.10358107409754146No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4610.10358107409754146No Hit
ACTCACAACTATGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4550.10223294731969929No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT4540.10200825952339225No Hit
CGTTAGATGTGTCTGCGGCGTTTTATCATATTCCTCTGCATCCTGCTGCT4540.10200825952339225No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4540.10200825952339225No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4520.1015588839307782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)