FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445062
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA15380.3455698307202143No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12790.2873756914766931No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10960.24625782475250638No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10780.24221344441897985No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10150.22805811325163683No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10130.2276087376590228No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9880.22199154275134703No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9670.21727309902889935No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9390.21098184073230244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9200.20671277260246884No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8610.19345619262035402No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8360.18783899771267823No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8260.1855921197496079No Hit
GGTTCTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTG8070.18132305161977433No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7990.17952554924931807No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7860.17660460789732665No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7730.17368366654533526No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7700.17300960315641417No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7550.16963928621180868No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7390.16604428147089617No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7340.16492084248936104No Hit
CCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGC7200.1617752133410626No Hit
GTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC7100.15952833537799227No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6880.1545852038592376No Hit
CCCAAAACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGC6850.1539111404703165No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6830.15346176487770244No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGCAGTAA6640.14919269674786884No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6510.14627175539587745No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6450.14492362861803523No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6410.14402487743280712No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGAACCACATC6350.14267675065496493No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6270.14087924828450868No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6200.13930643371035945No Hit
CTTCCAGGGGACCTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGT6120.13750893133990322No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6110.13728424354359617No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6030.1354867411731399No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT5890.13234111202484147No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5870.1318917364322274No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGCAGT5850.13144236083961336No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5850.13144236083961336No Hit
CATCCAACGATAACCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC5650.12694860491347273No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5650.12694860491347273No Hit
CTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5360.12043265882056883No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5300.11908453204272663No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5190.11661296628334929No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5170.11616359069073522No Hit
GTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAACAGTTGTCT5150.11571421509812116No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5140.11548952730181412No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5130.11526483950550709No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTCTGGGGGC5030.11301796154243678No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5020.11279327374612974No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5010.1125685859498227No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4980.11189452256090164No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4960.11144514696828757No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4930.11077108357936648No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4930.11077108357936648No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4910.11032170798675242No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCAAGGAGATCTCGAA4880.10964764459783131No Hit
GTATGGATCGGCAGAGGAGACACGAAGGTTCCACGCATGCGCTGATGGCC4860.10919826900521726No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4790.10762545443106802No Hit
ATAAAATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAG4740.10650201544953287No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4730.10627732765322584No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4630.10403044969015551No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG4590.10313169850492741No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4590.10313169850492741No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4520.1015588839307782No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4460.100210757152936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)