FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356548
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT18980.5323266432570088No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG17820.49979245431190195No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA17090.47931835264817074No Hit
AAATCAGACAACTTTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA15780.44257715651188617No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT14980.42013978482560554No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12630.35423000549715605No Hit
AATTTGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT12590.35310813691284204No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG11790.33067076522656136No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11530.32337861942852014No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11280.3163669407765574No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10100.2832718175392935No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATA9990.2801866789324299No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8900.24961576000987243No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8530.23923847560496764No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8490.2381166070206536No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8390.23531193555986854No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG8250.2313853955147694No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT8230.23082446122261235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7800.21876437394123652No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7530.21119176099711678No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7410.2078261552441747No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA7230.20277774661476156No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7160.200814476592212No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6860.19240046220985674No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6760.18959579074907165No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6610.18538878355789404No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6590.184827849265737No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT6550.183705980681423No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6390.17921850634416686No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6360.1783771049059313No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6230.1747310320069107No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG6220.17445056486083219No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6210.1741700977147537No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6140.17220682769220413No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6120.17164589340004713No Hit
ACTGCTTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC5980.167719353354948No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5940.16659748477063396No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5930.16631701762455547No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5810.16295141187161336No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5750.1612686089951423No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5710.16014674041082827No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5660.15874440468043574No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5650.15846393753435722No Hit
GGGGCACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGT5630.15790300324220022No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5510.1545373974892581No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5480.15369599605102258No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5420.15201319317455153No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5360.15033039029808049No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5260.1475257188372954No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5250.14724525169121688No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5170.14500151452258883No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT5150.1444405802304318No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5140.1441601130843533No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5130.1438796459382748No Hit
GGCACAGACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGT5050.14163590876964674No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC5040.14135544162356822No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5030.1410749744774897No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5000.14023357303925418No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4990.13995310589317567No Hit
GTCTAATTTTTAGGCCCATATTAACGTTGACATAGCTGACTACTGATTCC4950.13883123730886165No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4950.13883123730886165No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4900.1374289015784691No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4890.13714843443239058No Hit
GGGAATCAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGA4870.13658750014023358No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4870.13658750014023358No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4820.13518516440984102No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGC4800.134624230117684No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4800.134624230117684No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG4730.13266096009513445No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4730.13266096009513445No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4720.13238049294905593No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4680.13125862436474192No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4650.1304172229265064No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4520.12677115002748576No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4500.12621021573532876No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA4480.12564928144317175No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC4440.12452741285885771No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG4400.12340554427454369No Hit
GTTGACGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCAT4380.12284460998238667No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4370.12256414283630815No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4360.12228367569022965No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4350.12200320854415113No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4290.12032040566768008No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4280.12003993852160158No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4190.117515734206895No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4170.11695479991473798No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4120.11555246418434545No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4110.11527199703826693No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4070.11415012845395289No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4070.11415012845395289No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4050.11358919416179589No Hit
ATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACCAGCACCGG4020.11274779272356036No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTTTTGTGGTTTCACATTTCCT3940.1105040555549323No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3930.11022358840885378No Hit
GTCTAATTTTTAGGCCCATATTAACGTTGACATAGCTGACTACTGCTTCC3890.10910171982453974No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3870.10854078553238275No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG3850.10797985124022572No Hit
ATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTAA3850.10797985124022572No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3850.10797985124022572No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA3830.1074189169480687No Hit
GTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGCC3800.10657751550983319No Hit
GGGAATCAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA3780.10601658121767617No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA3780.10601658121767617No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3770.10573611407159765No Hit
CTTTAGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGGA3740.10489471263336213No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC3740.10489471263336213No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3730.10461424548728361No Hit
GGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGCAGCTC3720.10433377834120511No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3710.10405331119512662No Hit
ATTCTGTGTTGGGGTGAGTTGACGAATCTAGCCACCTGGGTGGGAAGTAA3700.1037728440490481No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC3690.10349237690296959No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT3680.10321190975689108No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3670.10293144261081257No Hit
GGGCCACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATA3650.10237050831865555No Hit
CTATTCTGTGTTGGGGTGAGTTGACGAATCTAGCCACCTGGGTGGGAAGT3650.10237050831865555No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3650.10237050831865555No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3630.10180957402649853No Hit
CTCCAAAAGATACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3590.1006877054421845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)