FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542216
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24730.4560912994083539No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT21750.40113165233043657No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG19670.3627705563834339No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT14930.27535152042728356No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13900.25635540079968133No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12800.23606828275078567No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11440.21098602770851468No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11210.20674417575283652No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10620.1958629033447925No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10450.1927276214645086No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT9890.18239963409416174No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTT9830.18129306401876744No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCGCAG8590.15842394912728505No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8580.15823952078138603No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8030.1480959617569382No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT7980.14717382002744295No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC7790.14366968145536096No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7730.14256311137996666No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7710.14219425468816857No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7530.13887454446198563No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7390.1362925476193989No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7350.13555483423580272No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT6970.12854655709163876No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6800.1254112752113549No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6800.1254112752113549No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA6690.12338256340646532No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6620.12209156498517197No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6380.11766528468359473No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6360.11729642799179663No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTGTTCG6300.11618985791640231No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC6290.11600542957050328No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6270.11563657287870517No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5780.10659958392965166No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5760.10623072723785355No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5730.1056774422001564No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5670.10457087212476208No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5660.10438644377886305No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG5590.10309544535756968No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5580.10291101701167063No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5500.10143559024447821No Hit
ATGTTAATATGGGCCTGAAGATCAGACAACTATTGTGGTTTCACATTTCC5490.10125116189857916No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5440.10032902016908392No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG5430.10014459182318486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)