FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542216
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG29580.5455390471693937No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT21620.398734083833749No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG20510.3782625374389542No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14000.25819968425867185No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT13940.2570931141832775No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13620.2511914071145079No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTCCCAAAAGTAAGA13130.24215441816545438No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11630.21449016628059667No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11120.20508432063974505No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10200.18811691281703233No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTT9310.17170279003201677No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCGCAG9200.1696740782271272No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT8990.1658010829632471No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8440.1556575239387993No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8430.15547309559290023No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8250.15215338536671733No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7680.1416409696504714No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7600.14016554288327898No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7510.13850568777018754No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT7390.1362925476193989No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7340.13537040588990365No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA7150.1318662673178217No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7150.1318662673178217No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6900.12725555867034538No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC6800.1254112752113549No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6750.12448913348185964No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT6610.12190713663927291No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC6390.11784971302949378No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTGTTCG5980.11028815084763267No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5940.10955043746403648No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5870.10825943904274311No Hit
ATGTTAATATGGGCCTGAAGATCAGACAACTATTGTGGTTTCACATTTCC5840.10770615400504596No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5720.10549301385425734No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5630.10383315874116587No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG5560.10254216031987252No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5530.10198887528217537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)