FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534671
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG32030.5990599826809384No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23200.4339116952294027No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18720.3501218506333802No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18640.34862560340845117No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT16840.31496004084754925No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT16830.31477300994443314No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA16700.31234160820392354No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14880.2783019838367894No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14620.2734391803557702No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG12030.22499817644869463No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCTCTGACATACT11940.22331489832064952No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA11820.22107052748325606No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11780.22032240387079155No Hit
GCCTAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGTCTAT11390.21302819864926278No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT10970.20517290071838568No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA10440.19526026285323125No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGGTAGCCACATTGTGTAAAA10130.18946230485663146No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9690.1812329451195221No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9630.18011075970082538No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9240.1728165544792966No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC9030.16888890551385807No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8870.1658964110640001No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.16290391661414214No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8510.1591632985518197No Hit
CTCCAAAAGACACCAAATACTCAAGCACAGTTTCTCTTCCAAAAGTAAGA8410.15729298952065848No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT8300.15523564958638117No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC8210.15355237145833606No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8140.1522431551365232No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC7830.14644519713992343No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT7760.14513598081811058No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7730.1445748881087622No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7520.14064723914332364No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT7430.13896396101527855No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC7360.1376547446934657No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA7230.13522334295295613No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7220.13503631204983998No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7160.13391412663114327No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7130.1333530339217949No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC7070.13223084850309816No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7040.1316697557937498No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6850.12811616863454348No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6820.1275550759251951No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6650.12437555057222105No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6620.12381445786287268No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA6570.12287930334729208No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTATT6520.12194414883171145No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6440.12044790160678248No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6350.11876462347873738No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGC6280.11745540715692453No Hit
CTTTTACACAATGTGGCTACCCTGCCTTGATGCCTTTATATGCATGTATA6230.11652025264134393No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACTGAGT6210.11614619083511168No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6090.11390181999771823No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG6040.1129666654821376No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5960.11147041825720863No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5910.11053526374162803No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5900.1103482328385119No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5760.1077298001948862No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5650.10567246026060885No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5610.10492433664814438No Hit
CTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAG5560.10398918213256375No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5550.10380215122944765No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5430.10155778039205418No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5430.10155778039205418No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5360.10024856407024132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)