FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595677
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA44600.7487279179823965No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT42400.7117951507276595No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG36850.6186238515168456No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA32920.5526484991027016No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT32690.5487873461624336No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT30650.5145405983444048No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG30540.512693959981668No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC30200.5069861686786631No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA24060.4039101727949879No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG23150.3886334372487103No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC22620.37973599786461454No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22220.3730209492728442No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG21890.3674810341846336No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA21470.3604302331632747No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG21020.35287580349753306No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT20990.3523721748531503No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG20440.343138983039466No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT20080.3370954393068727No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC19760.3317234004334564No Hit
TTATGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA18820.315943036242796No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC18600.3122497595173223No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA18330.3077171017178773No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA17720.2974766526154275No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA17300.2904258515940686No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA17140.28773983215736043No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA16980.28505381272065233No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC16590.27850664034367617No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA16590.27850664034367617No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG16560.2780030116992934No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG16470.27649212576614507No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA16240.2726309728258771No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT16230.27246309661108287No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15440.2592008756423364No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT14630.24560290224400136No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA14580.24476352117003006No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT14350.24090236822976208No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA13780.23133342398648934No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13780.23133342398648934No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13360.22428262296513043No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT13250.22243598460239358No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA13240.2222681083875993No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA12800.2148815549366519No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT12710.21337066900350357No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA12280.20615199176735044No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT12020.20178721018269968No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11800.19809393345722598No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT11780.19775818102763745No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG11760.19742242859804893No Hit
ATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTA11760.19742242859804893No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT11540.19372915187257522No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11470.19255401836901542No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT11410.19154676108024987No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTT11330.1902037513618958No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC11170.18751773192518764No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT11060.1856710935624508No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT10980.1843280838440967No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG10550.17710940660794358No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10490.176102149319178No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC10340.17358400609726413No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG9940.16686895750549377No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9870.16569382400193394No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGC9740.16351143320960856No Hit
CCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTT9720.16317568078002004No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9480.1591466516249578No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT9430.1583072705509865No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATA9420.15813939433619226No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG9300.15612487975866116No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG8880.14907407873730227No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8860.14873832630771375No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA8800.1477310690189482No Hit
TTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG8520.14303053500470891No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8520.14303053500470891No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT8450.14185540150114911No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT8410.1411838966419721No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT8400.1410160204271778No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG8260.13866575342005819No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC8210.13782637234608688No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT8170.13715486748690986No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG8100.13597973398335003No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8090.13581185776855578No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8040.13497247669458448No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7960.1336294669762304No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG7930.13312583833184763No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7910.1327900859022591No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7830.13144707618390503No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA7790.130775571324728No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA7770.13043981889513948No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7680.12892893296199115No Hit
GGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7660.12859318053240262No Hit
AACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAAT7440.12489990380692892No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7390.12406052273295763No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7170.12036724600748391No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7140.11986361736310114No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGACC7030.11801697900036429No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT6860.11516308334886188No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6850.11499520713406763No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG6790.11398794984530207No Hit
TTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGA6770.11365219741571356No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6740.11314856877133077No Hit
CAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6700.11247706391215373No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT6690.11230918769735948No Hit
CCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAA6570.11029467311982837No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6530.10962316826065133No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6450.10828015854229725No Hit
GTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6300.10576201532038335No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTA6280.10542626289079485No Hit
TGCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACA6250.10492263424641207No Hit
CAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGT6230.10458688181682356No Hit
ATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT6220.1044190056020293No Hit
GTACTTTACCGCAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGA6200.10408325317244077No Hit
GATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC6130.10290811966888096No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC6030.10122935752093837No Hit
CGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGG6010.10089360509134984No Hit
CCACAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAAT6010.10089360509134984No Hit
CATTTAAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6000.10072572887655559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)