FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595677
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA43280.7265682576295542No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT36670.6156020796505489No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG34350.5766547978182807No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT33470.561881690916386No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG32000.5372038873416297No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA31530.5293137052462995No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT29620.4972493482205961No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC28730.48230836510390696No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA27330.4588056950327107No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG23900.40122415335827977No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG23270.3906479518262414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22140.3716779395544901No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC20540.3448177451874086No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG20540.3448177451874086No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG20450.3433068592542603No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT20300.3407887160323464No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA20230.33961358252878654No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC19910.3342415436553703No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC19500.3273586188488056No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA19090.320475694042241No Hit
TTATGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA18350.30805285414746586No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA18310.3073813492882888No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT18200.30553471092555196No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA17280.2900900991644801No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA16580.2783387641288819No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT16560.2780030116992934No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG16420.27565274469217377No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA15880.26658742909328376No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA15800.2652444193749297No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG15720.2639014096565756No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA15530.2607117615754847No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA15260.2561791037760397No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT15010.25198219840618324No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14870.2496319313990636No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA13590.22814377590539842No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT13520.2269686424018386No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT13430.22545775646869026No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC12950.2173996981585658No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT12870.21605668844021172No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA12640.21219553549994374No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA12470.20934163984844137No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT12390.2079986301300873No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG12050.20229083882708246No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11750.19725455238325468No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11510.19322552322819245No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG11450.19221826593942692No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11310.18986799893230727No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT11310.18986799893230727No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT11260.189028617858336No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT11110.1865104746364221No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11080.18600684599203932No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC11070.18583896977724504No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG10780.18097055954821153No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC10720.17996330225944596No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTT10300.17291250123808707No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATA10250.17207312016411577No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC10120.16989072937179042No Hit
ATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTA9960.16720470993508227No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9810.1646865667131684No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT9740.16351143320960856No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA9670.16233629970604874No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGC9660.1621684234912545No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG9320.15646063218824965No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8890.14924195495209652No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT8580.14403779229347446No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8210.13782637234608688No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA8180.1373227437017041No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG8170.13715486748690986No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC8090.13581185776855578No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8080.1356439815537615No Hit
CCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTT8050.13514035290937873No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT7990.13413309562061318No Hit
TTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG7930.13312583833184763No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7890.13245433347267058No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT7870.13211858104308208No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7800.13094344753952225No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG7760.13027194268034523No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7580.12725017081404855No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA7510.12607503731048875No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7480.12557140866610597No Hit
GGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7370.1237247703033691No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7330.12305326544419207No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7290.12238176058501503No Hit
GTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7270.12204600815542652No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA7260.12187813194063225No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7240.12154237951104373No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGACC7190.12070299843707244No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7140.11986361736310114No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT7100.1191921125039241No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG7080.11885636007433557No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG7000.11751335035598152No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6990.11734547414118726No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG6930.11633821685242171No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT6790.11398794984530207No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6760.1134843212009193No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6670.11197343526777095No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6640.11146980662338818No Hit
GTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6640.11146980662338818No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6430.10794440611270872No Hit
AACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAAT6400.10744077746832595No Hit
CAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6390.10727290125353169No Hit
TTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGA6230.10458688181682356No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6170.103579624528058No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6120.1027402434540867No Hit
ATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTC6100.10240449102449818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)