FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392695
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG23500.5984288060708692No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13720.3493805625230777No Hit
ATCCTAATCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAGCCGTAT10490.26712843300780503No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9300.23682501687059934No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8520.21696227352016195No Hit
GAAAAACGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAATCT8240.2098320579584665No Hit
GGATAATACGGCTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATT8090.20601229962184392No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7760.19760883128127427No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7650.19480767516775105No Hit
GTATACATGCATACAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7510.19124256738690332No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA7250.18462165293675753No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA7150.18207514737900915No Hit
CTCCAAAAGATACCAAATACTCAAGTACCGTTTCTCTTCCAAAAGTAAGA7070.18003794293281045No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG7010.17851003959816142No Hit
GATTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6910.17596353404041304No Hit
GTTCTATCCTAATCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAGC6900.1757088834846382No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6580.16756006569984339No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6550.16679611403251887No Hit
CCACAAAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCAT6500.16552286125364468No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6420.16348565680744598No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6420.16348565680744598No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6370.1622124040285718No Hit
ACGTTGGGGCTACTCCCTTAATTTCATGGGATATGTAATTGGAAGTTGGG6160.1568647423573002No Hit
GCCCAATAGACCCACAATCCTTTGACATACTTTCCAATCAATAGGTCTAT6120.15584614013420084No Hit
CTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT6110.155591489578426No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGG5980.1522810323533531No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG5970.15202638179757827No Hit
CCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCAT5860.14922522568405505No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5840.14871592457250538No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATATT5370.136747348451088No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5280.13445549344911445No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5280.13445549344911445No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTTCCAATTTTCTT5180.13190898789136607No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTGTATGCATGTATA5110.1301264340009422No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5050.12859853066629318No Hit
AAAACGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAATCTTA5000.12732527788741899No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4980.1268159767758693No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4930.12554272399699512No Hit
TAATACGGCTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATTAGG4910.12503342288544544No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGCTTAATGCC4830.12299621843924674No Hit
GTTCAGGATAATACGGCTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4740.1207043634372732No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4700.11968576121417385No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4700.11968576121417385No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4610.1173939062122003No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4530.11535670176600162No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4390.11179159398515387No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTTCCAATTTTCTTTTGTC4340.1105183412062797No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4340.1105183412062797No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4330.11026369065050484No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4330.11026369065050484No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC4310.10975438953895517No Hit
AGCGATAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGTA4290.10924508842740548No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4230.10771718509275646No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4230.10771718509275646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4220.10746253453698164No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4170.10618928175810743No Hit
CTCCAAAAGATACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA4150.10567998064655777No Hit
CATTTAAACCCAAATAAAACCAAACGGTGGGGCTACTCCCTTAATTTCAT4100.10440672786768358No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4010.10211487286571003No Hit
GGATAATATGGCTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATT4010.10211487286571003No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3990.10160557175416035No Hit
AATACGGCTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATTAGGA3970.10109627064261069No Hit
ATGTAAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC3970.10109627064261069No Hit
TGGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG3970.10109627064261069No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTTAAA3930.10007766841951132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)