FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609538
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28920.474457704031578No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20270.3325469453914276No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA20060.3291017131007419No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17530.2875948669320042No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT17190.2820168717947035No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16520.27102494020061096No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16230.2662672384658545No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15790.2590486565234653No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG15430.2531425440251469No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT15170.24887701833191697No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14790.2426427884725809No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA12720.20868264160725006No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCGTAT12590.2065498787606351No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12480.2047452332750378No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA12240.20080782494282554No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA11300.18538630897499417No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11140.18276137008685267No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10980.18013643119871114No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG10900.1788239617546404No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10650.17472249474191928No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA10630.1743943773809016No Hit
GTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGTACAATA10310.16914449960461858No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10300.16898044092410974No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10100.16569926731393286No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10090.165535208633424No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9810.16094156557917635No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCAT9650.15831662669103486No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA9630.15798850933001718No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA9580.15716821592747293No Hit
GGTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGTACAAT9320.152902690234243No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC9300.1525745728732253No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9250.15175427947068107No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC9140.1499496339850838No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9090.14912934058253957No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8800.14437163884778306No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8770.14387946280625655No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT8340.13682493954437625No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC8220.1348562353782701No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8220.1348562353782701No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGT8150.13370782461470818No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8150.13370782461470818No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8020.13157506176809322No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8010.13141100308758435No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7900.12960635760198708No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7710.12648924267231904No Hit
GGAACATATTGTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAA7640.1253408319087571No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7610.1248486558672306No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7580.12435647982570405No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAATTCG7560.12402836246468638No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA7410.1215674822570537No Hit
GGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG7390.121239364896036No Hit
ATATTGTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAAATAGA7280.11943471941043872No Hit
GGATAATACGGTTTAATGCCCTTGTCCAAGGGCAAATATTTGGTAAGGTT7280.11943471941043872No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7270.11927066072992987No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7160.11746601524433259No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG7120.11680978052229722No Hit
ATGTAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC7050.1156613697587353No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6980.11451295899517339No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6840.11221613746804958No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6730.11041149198245227No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6700.10991931594092576No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6680.10959119857990805No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG6660.10926308121889036No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6660.10926308121889036No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6650.10909902253838154No Hit
GTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAAATAGACCTAT6620.108606846496855No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6580.10795061177481963No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA6560.10762249441380192No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6510.1068022010112577No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6420.1053256728866781No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6360.10434132080362503No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA6350.1041772621231162No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6330.10384914476209851No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC6310.10352102740108081No Hit
CTTTTGGAGTGTGGATACGCACTCCTCCCGCTTACAGACCACCAAATGCC6280.1030288513595543No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6210.10188044059599238No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA6180.10138826455446584No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6140.10073202983243046No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6110.10023985379090394No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACAT6110.10023985379090394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)