FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609538
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28860.47347335194852497No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19490.31975036831173775No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA19450.3190941335897024No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18940.3107271408837513No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18790.3082662606761186No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA17030.279391932906562No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16160.26511882770229256No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT15770.25872053916244764No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA15660.2569158936768503No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG14620.23985379090393052No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13030.21376846070302424No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA12300.20179217702587862No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11980.19654229924959563No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA11970.19637824056908676No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG11750.19276894959789218No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCGTAT11680.19162053883433028No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA11660.19129242147331257No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11630.19080024543178606No Hit
GTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGTACAATA10670.175050612102937No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA10590.17373814265886622No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10550.17308190793683087No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10270.1684882648825832No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10130.16619144335545938No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9990.16389462182833556No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9940.16307432842579134No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9770.16028533085714097No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA9670.15864474405205253No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC9460.1551995117613668No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9180.15060586870711917No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9150.15011369266559263No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA9010.1478168711384688No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCAT8980.14732469509694227No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8930.14650440169439805No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8780.14404352148676539No Hit
GGTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGTACAAT8680.14240293468167695No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8460.13879364371048236No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT8420.138137408988447No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG8390.13764523294692046No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8370.13731711558590276No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8320.13649682218335854No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC8240.1351843527392878No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7840.12862200551893402No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA7810.12812982947740748No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACAGTGCTTGAGTTTTAGT7800.12796577079689864No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7790.1278017121163898No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7790.1278017121163898No Hit
ATATTGTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAAATAGA7770.1274735947553721No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7740.12698141871384558No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7640.1253408319087571No Hit
GGAACATATTGTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAA7560.12402836246468638No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7550.12386430378417752No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA7170.11763007392484144No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6960.11418484163415571No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAATTCG6940.11385672427313802No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6930.11369266559262917No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6890.1130364308705938No Hit
GGATAATACGGTTTAATGCCCTTGTCCAAGGGCAAATATTTGGTAAGGTT6860.11254425482906726No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG6790.11139584406550535No Hit
GTACTAAAACTCAAGCACTGTTTTCGAAAATTGCCTGTAAATAGACCTAT6760.11090366802397882No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6700.10991931594092576No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6640.10893496385787269No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6620.108606846496855No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6610.10844278781634614No Hit
CTTTTGGAGTGTGGATACGCACTCCTCCCGCTTACAGACCACCAAATGCC6600.10827872913583729No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6580.10795061177481963No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAAC6540.10729437705278423No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA6420.1053256728866781No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6400.10499755552566042No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6400.10499755552566042No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6360.10434132080362503No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT6300.10335696872057197No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6290.10319291004006315No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA6240.1023726166375189No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6190.10155232323497468No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT6170.101224205873957No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6140.10073202983243046No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6120.10040391247141277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)