FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373110
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29210.7828790437136501No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19930.5341588271555305No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17840.4781431749350058No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA15470.41462303342177914No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12000.3216209696872236No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11110.29776741443542115No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10180.27284178928466135No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10000.268017474739353No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC9270.24845219908338026No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9030.24201977968963578No Hit
CTCCAAAAGATACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8900.23853555251802416No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8440.22620674868001392No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8370.22433062635683845No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8270.22165045160944497No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8190.2195063118115301No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7820.20958966524617403No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAATAATGATTAACTACA7740.20744552544825923No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7660.2053013856503444No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7340.1967248264586851No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA7260.1945806866607703No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7100.19029240706494063No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7100.19029240706494063No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7050.18895231969124388No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6960.1865401624185897No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6850.18359197019645682No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6740.18064377797432393No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6570.17608748090375492No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6540.17528342847953687No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6340.16992307898474981No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6320.1693870440352711No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6310.16911902656053174No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6310.16911902656053174No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA6300.1688510090857924No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5970.16000643241939375No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5860.15705824019726086No Hit
GATTAATTATGCCTGCTAGGTTTTATCCCAATGTTACTAAATATTTGCCC5750.15411004797512798No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5700.15276996060143122No Hit
GTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5670.15196590817721317No Hit
GTATTATCCAGAGTATGTAGTTAATCATTATTTCCAGACGCGACATTATT5590.1498217683792983No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATATTCAA5560.1490177159550803No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5520.14794564605612287No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5460.14633754120768674No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAGATTAATTATGCCTGCTAGG5440.14580150625820804No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5430.14553348878346867No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5320.14258529656133578No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5250.14070917423816032No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5230.1401731392886816No Hit
GGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5150.1380289994907668No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAAT5110.13695692959180938No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5100.13668891211707002No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT5030.13481278979389455No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5030.13481278979389455No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5020.1345447723191552No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5010.13427675484441584No Hit
CCCCAAAAGACCTACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT4960.13293666747071908No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG4880.13079252767280428No Hit
GTTTACTAGTACCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4780.12811235292541073No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4750.12730830050119268No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4740.12704028302645332No Hit
ATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGATTGACGA4730.12677226555171398No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTC4710.12623623060223527No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4660.12489614322853851No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTAGGTCT4650.12462812575379915No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT4620.12382407332958108No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4610.12355605585484172No Hit
GTATGGATCGGCAGAGGAGACCCAAAGGTTCCACGCATGCGCTGATGGCC4520.12114389858218755No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4510.1208758811074482No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT4480.12007182868323016No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGACCCGTAT4460.11953579373375145No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4450.11926777625901208No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4440.11899975878427273No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4430.11873174130953337No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4410.11819570636005466No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4350.11658760151161855No Hit
TGGAAAACATCGCATCAGGACTCCTAGGACCCCTGCTCGCGTTACAGGCG4350.11658760151161855No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4330.11605156656213984No Hit
CCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG4310.11551553161266113No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4290.11497949666318245No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTAGGTCTTTTGGGGTTT4280.1147114791884431No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4240.11363940928948567No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC4220.11310337434000696No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4180.11203130444104956No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4180.11203130444104956No Hit
GTGTAAATAATGTCGCGTCTGGAAATAATGATTAACTACATACTCTGGAT4170.1117632869663102No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4170.1117632869663102No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4160.11149526949157085No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4150.1112272520168315No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4150.1112272520168315No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4110.11015518211787408No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA4070.10908311221891667No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA4030.10801104231995926No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4030.10801104231995926No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4020.1077430248452199No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4020.1077430248452199No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCTACAATTCG4020.1077430248452199No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC4000.1072069898957412No Hit
GAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC3990.10693897242100185No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3960.10613491999678379No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3950.10586690252204443No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3930.10533086757256573No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTA3900.10452681514834768No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC3890.10425879767360832No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3850.1031867277746509No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3780.10131060545147544No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3780.10131060545147544No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3780.10131060545147544No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3770.10104258797673608No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3770.10104258797673608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)