FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571011
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23020.4031445979149263No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21440.3754743779016516No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18400.32223547357231297No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC17030.29824294102915705No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT16020.28055501557763335No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA15350.26882144126820673No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG14540.25463607531203425No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14120.2472807003717967No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13950.24430352480074813No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG13730.2404507093558618No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA13140.23011815884457568No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA12620.22101150415666246No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA12300.21540740896410054No Hit
GTCCGGTGCTGGTCGAATTTGATCCTGGAATTACAGGACAAACGGGCAAC11780.20630075427618733No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGA11650.20402409060420904No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT11000.19264077224431753No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10660.18668642110222045No Hit
GTTGTGAGTAGGCCCTGAGACTGAGGGCTCCACCCCAACAGTCCCCCATG10480.18353411755640434No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10350.18125745388442605No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9830.17215079919651285No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9700.16987413552453454No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9350.16374465640766991No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9320.16321927248336723No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC9200.16111773678615648No Hit
AAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC9150.16024209691231867No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9130.15989184096278355No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9080.15901620108894576No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCT9070.1588410731141782No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCGCCAAACTCTTCAAG9050.15849081716464306No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8980.15726492134127013No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8740.15306184994684865No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8680.1520110820982433No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8620.15096031424963793No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA8350.14623185893091378No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8320.1457064750066111No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGA8320.1457064750066111No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8300.14535621905707596No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8090.14167853158695717No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7950.1392267399402113No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7800.13659982031869788No Hit
CTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7690.1346734125962547No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7580.13274700487381155No Hit
GTTCAGATACTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAA7560.13239674892427641No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7470.13082059715136835No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC7310.12801854955508737No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC7290.12766829360555226No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7230.12661752575694687No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7170.12556675790834151No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7140.12504137398403883No Hit
CCTTCGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGG7120.12469111803450371No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA7090.12416573411020103No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC7080.12399060613543346No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6950.12171394246345515No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6850.11996266271577956No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6830.11961240676624442No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6770.11856163891763906No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6670.11681035916996346No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6660.1166352311951959No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT6640.11628497524566077No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6460.11313267169984467No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6400.11208190385123928No Hit
GTTGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG6330.11085600802786637No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT6240.10927985625495831No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6220.10892960030542317No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6140.1075285765072827No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6100.10682806460821244No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5900.10332550511286123No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5900.10332550511286123No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5830.10209960928948829No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5830.10209960928948829No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5790.10139909739041804No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5780.1012239694156505No Hit
TGATAGATGTGAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGGA5760.10087371346611536No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5750.1006985854913478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)