FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571011
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22960.40209383006632093No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21950.38440590461479723No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT18510.32416188129475615No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16190.28353219114868194No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15830.27722758405704967No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15800.276702200132747No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15160.2654940097476231No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA14900.26094068240366647No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT13500.23642276593620787No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA13260.23221969454178643No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12760.22346329580340835No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA12500.21890996845945174No Hit
GTCCGGTGCTGGTCGAATTTGATCCTGGAATTACAGGACAAACGGGCAAC12430.2176840726360788No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA11880.20805203402386296No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11560.202447938831301No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGA10730.18791231692559338No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10720.1877371889508258No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT10140.17757976641430726No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9980.17477771881802626No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT9710.1700492634993021No Hit
GTTGTGAGTAGGCCCTGAGACTGAGGGCTCCACCCCAACAGTCCCCCATG9610.1682979837516265No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9490.16619644805441575No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9400.1646202962815077No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9300.1628690165338321No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG9240.16181824868522673No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGA9170.1605923528618538No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9110.15954158501324844No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC9090.1591913290637133No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9050.15849081716464306No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8920.15621415349266476No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8870.15533851361882697No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCGCCAAACTCTTCAAG8850.15498825766929183No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8160.1429044274103301No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8050.14097801968788692No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8020.14045263576358422No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA7970.13957699588974642No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCT7880.13800084411683838No Hit
GTTCAGATACTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAA7870.13782571614207081No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA7740.13554905247009252No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7720.13519879652055738No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7530.13187136499997373No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7460.1306454691766008No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC7400.12959470132799544No Hit
CCTTCGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGG7290.12766829360555226No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC7180.12574188588310908No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7110.12451599005973615No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT7060.12364035018589835No Hit
CTGTTTACTTAGAAAAGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT7030.12311496626159567No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6940.12153881448868761No Hit
AAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC6920.12118855853915247No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6870.12031291866531468No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6820.11943727879147688No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6740.11803625499333638No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6480.11348292764937978No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT6450.1129575437250771No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6380.11173164790170417No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT6330.11085600802786637No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6200.10857934435588806No Hit
TTCTCTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGG6170.10805396043158538No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG6170.10805396043158538No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6130.10735344853251513No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6130.10735344853251513No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6120.10717832055774758No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6080.10647780865867733No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC5970.10455140093623416No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5960.10437627296146659No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5910.1035006330876288No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5800.10157422536518561No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5790.10139909739041804No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5790.10139909739041804No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5740.10052345751658023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)