FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352810
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27780.7873926476006916No Hit
GGGACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA15400.43649556418468866No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12000.34012641365040674No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA10670.30242906947082No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAAAGTCCAA9920.28117116861766955No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC9270.2627476545449392No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9000.25509481023780506No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8580.24319038576004082No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8560.2426235084039568No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8220.2329865933505286No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7370.20889430571695813No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7040.19954082934157194No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6990.19812363595136193No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6850.19415549445877384No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6600.18706952750772368No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6300.17856636716646354No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6170.17488166435191746No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6130.17374790963974945No Hit
TCTTTGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCTC5930.16807913607890934No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5810.16467787194240527No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5770.16354411723023723No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATT5730.16241036251806923No Hit
ACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC5710.1618434851619852No Hit
TCCTAAAACACATTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTG5610.15900909838156516No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5550.1573084663133131No Hit
AGCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTT5500.1558912729231031No Hit
ACTCACAACCGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5480.15532439556701907No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC5200.14738811258184292No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5130.14540404183554886No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4980.1411524616649188No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4940.14001870695275076No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4910.13916839091862473No Hit
CTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGCT4840.1371843201723307No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4720.13378305603582663No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4720.13378305603582663No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4700.13321617867974264No Hit
GCTACTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAG4600.13038179189932259No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4540.12868115983107056No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4540.12868115983107056No Hit
CAACCGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGA4360.12357926362631445No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4230.11989456081176837No Hit
AGCCAAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTT4220.11961112213372638No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4180.11847736742155834No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4170.11819392874351635No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4160.11791049006547433No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4130.11706017403134832No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4120.11677673535330632No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4030.11422578725092825No Hit
ACCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTT3880.10997420708029819No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3830.10855701369008816No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3790.10742325897792013No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG3770.10685638162183611No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3740.1060060655877101No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGC3710.10515574955358407No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG3650.10345511748533205No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3640.10317167880729003No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT3630.10288824012924805No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3630.10288824012924805No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA3590.10175448541708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)