FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460242
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23600.5127737146979198No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCCGAGAGTCCAA15280.3319992525671277No Hit
TTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC13430.2918030079827569No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11910.25877690432424677No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10900.23683192755115787No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10750.23357277258485754No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10460.22727173965001024No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10410.22618535466124345No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG10320.22422986168146322No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9170.1992430069398273No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9110.19793934495330717No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8920.19381108199599342No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8840.19207286601396656No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8700.1890309880454196No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8400.1825126781128189No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8280.17990535413977862No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC8180.17773258416224508No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT8100.17599436818021824No Hit
GACCCTTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC7930.1723006592184112No Hit
TTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCT7760.16860695025660413No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC7730.16795511926334408No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA7400.16078497833748331No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC7370.16013314734422326No Hit
GACAAGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTA7340.1594813163509632No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7230.15709126937567627No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7020.1525284524228558No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6610.1436200955149682No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6590.1431855415194615No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6340.1377536165756276No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6310.13710178558236755No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6200.13471173860708063No Hit
CCTAGAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT6110.13275624562730043No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6020.13080075264752022No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGAATCTCCTCC5960.12949709066100007No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGC5890.12797615167672657No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5800.1260206586969464No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5720.12428244271491953No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5690.12363061172165947No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5590.12145784174412591No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5580.12124056474637256No Hit
GCAGACGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG5580.12124056474637256No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5580.12124056474637256No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA5510.11971962576209907No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5460.11863324077333229No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5320.1155913628047853No Hit
GGTCAGGTCTCTGCCAAGTGTTTGCTGATGCAACCCCCACTGGATGGGGC5320.1155913628047853No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5320.1155913628047853No Hit
ATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA5290.11493953181152523No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5290.11493953181152523No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5240.11385314682275846No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5150.11189765384297826No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGT5150.11189765384297826No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5100.11081126885421148No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC5070.11015943786095142No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5060.10994216086319805No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5030.10929032986993797No Hit
GGAACATATTGTACTGAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA5030.10929032986993797No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5010.10885577587443128No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4970.10798666788341786No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4890.106248451901391No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4890.106248451901391No Hit
CAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA4780.1038584049261041No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTCAGTACAATA4770.10364112792835074No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4650.10103380395531046No Hit
TAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCC4650.10103380395531046No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA4650.10103380395531046No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4630.10059924995980375No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC4630.10059924995980375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)